Characterization of oqxAB in Escherichia coli Isolates from Animals, Retail Meat, and Human Patients in Guangzhou, China

Frontiers in Microbiology
Jing WangJian-Hua Liu

Abstract

The purpose of this study was to investigate the prevalence and genetic elements of oqxAB among Escherichia coli isolates from animals, retail meat, and humans (patients with infection or colonization) in Guangzhou, China. A total of 1,354 E. coli isolates were screened for oqxAB by PCR. Fifty oqxAB-positive isolates were further characterized by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), S1-PFGE, genetic environment analysis, plasmid replicon typing, and plasmid sequencing. oqxAB was detected in 172 (33.79%), 60 (17.34%), and 90 (18.07%) E. coli isolates from animal, food, and human, respectively. High clonal diversity was observed among oqxAB-positive isolates. In 21 oqxAB-containing transformants, oqxAB was flanked by two IS26 elements in the same orientation, formed a composite transposon Tn6010 in 19 transformants, and was located on plasmids (33.3~500 kb) belonging to IncN1-F33:A-:B- (n = 3), IncHI2/ST3 (n = 3), F-:A18:B- (n = 2), F-:A-:B54 (n = 2), or others. Additionally, oqxAB was co-located with multiple resistance genes on the same plasmid, such as aac(6')-Ib-cr and/or qnrS, which were identified in two F-:A18:B- plasmids from pigs, and blaCTX-M-55, rmtB, fosA3, and floR, which were d...Continue Reading

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Citations

Dec 14, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Shaopeng XuMei Hong
Mar 6, 2019·Antimicrobial Resistance and Infection Control·Jun LiHaihong Hao
May 25, 2021·Frontiers in Microbiology·Man-Xia ChangHong-Xia Jiang
Jul 11, 2021·The Journal of Antibiotics·Cristiane Dos SantosOctávio Luiz Franco

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Datasets Mentioned

BETA
BX664015
KX246266
KM877269
KT335433
KU341381
pHXY0908

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Epi Info
MEGA
ClustalW
RAST

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