Characterization of Oral Microbiome and Exploration of Potential Biomarkers in Patients with Pancreatic Cancer.

BioMed Research International
Haiyang SunLingyun Zou

Abstract

Pancreatic cancer (PC) is highly malignant and lacks an effective therapeutic schedule, hence that early diagnosis is of great importance to achieve a good prognosis. Oral bacteria have been proved to be associated with pancreatic cancer, but the specific mechanism has not been comprehensively illustrated. In our study, thirty-seven saliva samples in total were collected with ten from PC patients, seventeen from benign pancreatic disease (BPD) patients, and ten from healthy controls (HC). The oral bacterial community of HC, PC, and BPD groups was profiled by 16S rDNA high-throughput sequencing and bioinformatic methods. As shown by Simpson, Inverse Simpson, Shannon and Heip, oral microbiome diversity of HC, BPD and PC groups is in increasing order with the BPD and PC groups significantly higher than the HC group. Principal coordinate analysis (PCoA) suggested that grouping by PC, BPD and HC was statistically significant. The linear discriminant analysis effect size (LEfSe) identified high concentrations of Fusobacterium periodonticum and low concentrations of Neisseria mucosa as specific risk factors for PC. Furthermore, predicted functions showed changes such as RNA processing and modification as well as the pathway of NOD-lik...Continue Reading

References

Feb 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·I Brook, E H Frazier
Oct 26, 1999·Archives of Oral Biology·H SakamotoC E Nord
Jun 21, 2003·The American Journal of Clinical Nutrition·Rachael Z Stolzenberg-SolomonDemetrius Albanes
Jun 25, 2003·Annals of Epidemiology·Philippe P HujoelNoel S Weiss
Jan 21, 2004·Journal of Gastroenterology and Hepatology·Jee-Eun KimKyoo Wan Choi
Jul 21, 2005·Journal of Bacteriology·Yiping W HanCheri X Deng
Nov 8, 2005·Journal of Clinical Microbiology·Jørn A AasFloyd E Dewhirst
Jan 18, 2007·Journal of the National Cancer Institute·Dominique S MichaudCharles S Fuchs
Jun 11, 2008·Clinical & Experimental Metastasis·Sarah Jane LuntRichard P Hill
Sep 29, 2009·Cell Biology and Toxicology·Elizabeth A MazzioKaram F A Soliman
Jan 1, 2010·Journal of Oral Microbiology·Jukka H Meurman
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Sep 10, 2011·Bioinformatics·Tanja Magoč, Steven L Salzberg
Oct 20, 2011·Genome Research·Mauro CastellarinRobert A Holt
Dec 14, 2011·Current Protocols in Bioinformatics·Justin KuczynskiRob Knight
Jul 13, 2013·Journal of the National Cancer Institute·Brian M WolpinCharles S Fuchs
Aug 27, 2013·Nature Biotechnology·Morgan G I LangilleCurtis Huttenhower
Mar 7, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L FlanaganD J Hughes
Sep 12, 2014·Cell Host & Microbe·UNKNOWN Integrative HMP (iHMP) Research Network Consortium
Nov 28, 2014·Nucleic Acids Research·Michael Y GalperinEugene V Koonin
Mar 31, 2015·Microbiology·Guangyu LiuRachel M Exley
Dec 20, 2015·Oral Oncology·Leonardo Victor Galvão-Moreira, Maria Carmen Fontoura Nogueira da Cruz
Dec 31, 2015·Clinical and Experimental Medicine·Yun-Peng ZhaoChun-Fang Gao
May 10, 2016·Nature Reviews. Disease Primers·Jorg KleeffJohn P Neoptolemos
Mar 16, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Pourya GholizadehHossein Samadi Kafil
Jan 19, 2018·International Journal of Oncology·Kosuke TaketoKazuhiko Ogawa
Oct 31, 2018·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Erika Del CastilloDominique S Michaud
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jun 22, 2019·Science·Dannielle D EngleDavid A Tuveson

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Citations

May 31, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ziyu JiangJiege Huo
Aug 30, 2021·Cancer Metastasis Reviews·Jenny Jing LiWen Wee Ma

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Datasets Mentioned

BETA
SRP237984

Methods Mentioned

BETA
16S sequencing

Software Mentioned

NMDS
LEfSe
USEARCH
FLASH
PICRUSt
QIIME
ANNOSIM

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