Characterization of oral polymorphonuclear neutrophils in periodontitis patients: a case-control study

BMC Oral Health
Elena A NicuBruno G Loos

Abstract

Maintaining oral health is a continuous and dynamic process that also involves the immune system. Polymorphonuclear neutrophils (PMNs) migrate from blood circulation and become apparent in the oral fluid. Controversies exist regarding the specific role of the oral PMNs (oPMNs) in the presence of chronic oral inflammation, such as periodontitis. In this study we characterized cell counts, activation status, apoptosis, and reactive oxygen species (ROS) generation by oPMNs and circulatory (cPMNs), and the salivary protease activity, in subjects with and without periodontitis. Venous blood and oral rinse samples were obtained from 19 patients with untreated periodontitis and 16 control subjects for PMN isolation. Apoptosis and expression of cell activation markers CD11b, CD63, and CD66b were analyzed using flow cytometry. Constitutive ROS generation was detected using dihydrorhodamine123. Additionally, ROS production in response to stimulation was evaluated in samples incubated with 10 μM phorbol myristate acetate (PMA) or Fusobacterium nucleatum. Total protease activity was measured using substrate PEK-054. Periodontitis patients presented with over 4 times higher oPMN counts compared to controls (p = 0.007), which was a predictor...Continue Reading

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Citations

Oct 23, 2019·Journal of Immunology Research·Carolyn G J MoonenBruno G Loos
May 20, 2020·Revista da Associação Médica Brasileira·Gülbahar UstaogluMehmet İnanır
May 3, 2019·Immunopharmacology and Immunotoxicology·Shabir Ahmad Mir, Sadhna Sharma
Dec 14, 2018·Current Oral Health Reports·Patrick RijkschroeffElena A Nicu
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Jan 31, 2020·Frontiers in Immunology·Josefine Hirschfeld
May 28, 2019·BMC Oral Health·Martijn J L VerhulstBruno G Loos
Nov 1, 2020·Molecular Oral Microbiology·Irina Miralda, Silvia M Uriarte
Mar 30, 2021·Frontiers in Cellular and Infection Microbiology·Qingsong JiangMingyun Li
Apr 6, 2021·Frontiers in Cellular and Infection Microbiology·Jingyi WangMingyun Li
Apr 23, 2021·Oral Diseases·Julieta Saraí Becerra-RuizJuan Manuel Guzmán-Flores
Dec 7, 2021·Frontiers in Immunology·Bo YangYan Wang

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Methods Mentioned

BETA
biopsies
blood collection
PMA
flow cytometry
flowcytometric

Clinical Trials Mentioned

ISRCTN15252886

Software Mentioned

SPSS Statistics
Accuri CFlow Plus

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis