Characterization of Organic Solvent-Tolerant Lipolytic Enzyme from Marinobacter lipolyticus Isolated from the Antarctic Ocean

Applied Biochemistry and Biotechnology
Se Hyeon ParkHyung Kwoun Kim

Abstract

The Antarctic marine environment provides a good source of novel lipolytic enzymes that possess beneficial properties, i.e., resistance to extreme physical and chemical conditions. We found a lipolytic Escherichia coli colony that was transformed using genomic DNA from Marinobacter lipolyticus 27-A9 isolated from the Antarctic Ross Sea. DNA sequence analysis revealed an open reading frame of lipolytic enzyme gene. The gene translates a protein (LipA9) of 404 amino acids with molecular mass of 45,247 Da. Recombinant LipA9 was expressed in E. coli BL21 (DE3) cells and purified by anion exchange and gel filtration chromatography. The kcat/Km of LipA9 was 175 s-1 μM-1, and the optimum temperature and pH were 70 °C and pH 8.0, respectively. LipA9 had quite high organic solvent stability; it was stable toward several common organic solvents up to 50% concentration. Substrate specificity studies showed that LipA9 preferred a short acyl chain length of p-nitrophenyl ester and triglyceride. Sequence analysis showed that LipA9 contained catalytic Ser72 and Lys75 in S-x-x-K motif, like family VIII esterases. Homology modeling and site-directed mutagenesis studies revealed that Tyr141 and Tyr188 residues were located near the conserved mot...Continue Reading

References

Mar 5, 1992·Nature·R LüthyD Eisenberg
Sep 1, 1993·Protein Science : a Publication of the Protein Society·C Colovos, T O Yeates
Jul 17, 1999·Trends in Biotechnology·W Tischer, V Kasche
Jul 28, 1999·Applied Microbiology and Biotechnology·F NiehausG Antranikian
Sep 27, 2002·Current Opinion in Biotechnology·Karl-Erich Jaeger, Thorsten Eggert
May 5, 2006·Applied and Environmental Microbiology·C ElendW R Streit
May 23, 2007·Nucleic Acids Research·Markus Wiederstein, Manfred J Sippl
Feb 20, 1992·Biotechnology and Bioengineering·L A Gorman, J S Dordick
Jul 30, 2008·International Journal of Biological Macromolecules·K Sangeetha, T Emilia Abraham
Sep 13, 2011·Applied and Environmental Microbiology·Jeong Ho JeonJung-Hyun Lee
Dec 1, 2011·Journal of Microbiology and Biotechnology·Jin Yee Han, Hyung Kwoun Kim
Apr 7, 2012·Aquatic Biosystems·Ram KaranShiladitya Dassarma
Apr 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·K ThangaveluJ Sivaraman
Apr 30, 2013·Protein Science : a Publication of the Protein Society·Md Zahid KamalNalam Madhusudhana Rao
Mar 29, 2014·TheScientificWorldJournal·Shivika Sharma, Shamsher S Kanwar
Jan 15, 2016·Biological Procedures Online·Ashok KumarPankaj Kumar Arora

❮ Previous
Next ❯

Citations

Aug 28, 2020·Microbial Cell Factories·Prem ChandraPankaj Kumar Arora
Apr 8, 2021·Journal, Genetic Engineering & Biotechnology·Selfela Restu AdinaEsti Puspitasari
May 25, 2021·Journal of Microbiology and Biotechnology·Han Byeol Jeong, Hyung Kwoun Kim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.