Characterization of ori and parS-like functions in secondary genome replicons in Deinococcus radiodurans.

Life Science Alliance
Ganesh K Maurya, Hari S Misra

Abstract

The mechanisms underlying multipartite genome maintenance and its functional significance in extraordinary radioresistance of Deinococcus radiodurans are not well understood. The sequences upstream to parAB operons in chrII (cisII) and MP (cisMP) could stabilize an otherwise, non-replicative colE1 plasmid, in D. radiodurans DnaA and cognate ParB proteins bound specifically with cisII and cisMP elements. The ΔcisII and ΔcisMP cells showed the reduced copy number of cognate replicons and radioresistance as compared with wild type. Fluorescent reporter-operator system inserted in chrI, chrII, and MP in wild type and cisII mutants showed the presence of all three replicons in wild-type cells. Although chrI was present in all the ΔcisII and ΔcisMP cells, nearly half of these cells had chrII and MP, respectively, and the other half had the reduced number of foci representing these replications. These results suggested that cisII and cisMP elements contain both origin of replication and parS-like functions and the secondary genome replicons (chrII and MP) are maintained independent of chrI and have roles in radioresistance of D. radiodurans.

References

Aug 20, 1992·Journal of Molecular Biology·G T Marczynski, L Shapiro
Sep 1, 1981·Journal of Biochemistry· HarsojoA Matsuyama
Aug 30, 1996·The Journal of Biological Chemistry·K Tougu, K J Marians
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·J PoglianoD R Helinski
Jan 11, 2003·Science·Smadar Levin-ZaidmanAbraham Minsky
Jul 17, 2003·Molecular Microbiology·Ivy F LauDavid J Sherratt
Aug 28, 2003·Cell·Elizabeth S Egan, Matthew K Waldor
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Sep 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Matthew T G HoldenJulian Parkhill
Oct 2, 2004·Molecular Microbiology·Issay NarumiHiroshi Watanabe
Dec 23, 2004·Molecular Microbiology·Michael A Fogel, Matthew K Waldor
Oct 21, 2005·Journal of Bacteriology·Debasish PalDhruba K Chattoraj
Jan 18, 2006·Nature Reviews. Microbiology·Finbarr Hayes, Daniela Barillà
Feb 8, 2006·Biochemistry·Sharmistha Ghosh, Anne Grove
Apr 14, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuya MorikawaKunio Takeyasu
Dec 22, 2006·PloS One·Sebastian BreuertJörg Soppa
Oct 2, 2007·Journal of Bacteriology·Jonathan LivnyMatthew K Waldor
Dec 19, 2007·PLoS Genetics·Emilie EsnaultFrédéric Boccard
Jun 17, 2010·Cell·Kenn GerdesFlorian Szardenings
Jul 28, 2010·Molecular Microbiology·Anthony G VecchiarelliKiyoshi Mizuuchi
Jan 25, 2011·Journal of Bacteriology·Ryosuke KadoyaDhruba K Chattoraj
Jul 2, 2011·BMC Microbiology·Ramón Cervantes-RiveraMiguel A Cevallos
Aug 23, 2011·Current Opinion in Chemical Biology·Charles S McHenry
Sep 1, 2012·Annual Review of Genetics·Rodrigo Reyes-LamotheDavid J Sherratt

❮ Previous
Next ❯

Citations

Feb 25, 2021·The Journal of Biological Chemistry·Ganesh K MauryaHari S Misra

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR
fluorescence microscopy
gene knockouts
column chromatography
electrophoretic mobility shift assay

Software Mentioned

CellSens
Image J
oligo calc
Mellina II
WebLogo
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.