Abstract
Outer membranes were prepared from whole cells of various strains of Actinobacillus actinomycetemcomitans and analysed by SDS-polyacrylamide gel (12.5%) electrophoresis (SDS-PAGE). In all strains four common major outer membrane proteins (OMPs) with molecular masses of 30, 34, 36 and 39 kDa could be distinguished. Heating the OMP preparation of strain Y4 at 60, 70, 90 and 100 degrees C produced a band of 30 kDa, which gradually lost its intensity from 70 degrees C onwards concomitantly with the development of two new protein bands of 34 and 36 kDa. Furthermore, the 36 kDa OMP appeared susceptible to proteolysis by trypsin; degraded products apparently produced a new electrophoretic band of 27 kDa. Y4-derived OMP fractions were solubilized with a Triton-SDS mixture to investigate the presence of peptidoglycan-associated proteins. The 39 kDa OMP was found to be peptidoglycan-associated.
References
Mar 17, 1977·Biochimica Et Biophysica Acta·B LugtenbergR Peters
Sep 1, 1977·Journal of Clinical Pathology·J VandepitteP Jousten
Jan 1, 1987·Antonie van Leeuwenhoek·J M GriffissM A Apicella
Apr 1, 1989·Infection and Immunity·E J HansenE E Miller
Sep 1, 1986·Journal of Periodontal Research·T NishiharaS Hamada
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Nov 1, 1985·Journal of Clinical Microbiology·G M CarloneB D Plikaytis
Jan 1, 1985·Journal of Clinical Periodontology·J J Zambon
Sep 1, 1967·Neurology·B F MartinJ Ransohoff
Aug 15, 1970·Nature·U K Laemmli
Nov 15, 1984·Analytical Biochemistry·M R Loeb
May 1, 1982·Journal of Periodontal Research·P B RobertsonS C Holt
Sep 1, 1982·Journal of Periodontal Research·J SlotsR J Genco
Feb 1, 1984·Infection and Immunity·C C TsaiN S Taichman
Mar 21, 1983·Biochimica Et Biophysica Acta·B Lugtenberg, L Van Alphen
Jan 1, 1983·Infection and Immunity·J M Di Rienzo, E L Spieler
Jul 1, 1983·Infection and Immunity·J J ZambonR J Genco
Oct 12, 1984·Journal of Chromatography·I Brondz, I Olsen
Aug 1, 1983·Journal of Bacteriology·L van AlphenH C Zanen
Aug 1, 1982·Journal of Periodontology·T E Van DykeM J Levine
Jul 1, 1982·Infection and Immunity·A NowotnyF Sanavi
Dec 1, 1982·Infection and Immunity·B J ShenkerC C Tsai
Dec 1, 1980·Infection and Immunity·P Kiley, S C Holt
Citations
Apr 1, 1996·FEMS Microbiology Letters·T HattoriM Takigawa
Jan 21, 2000·FEMS Immunology and Medical Microbiology·S PajuS Asikainen
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
Jul 15, 2017·Journal of Proteome Research·Arancha Llama-PalaciosMariano Sanz
Jan 10, 1998·Infection and Immunity·P A WhiteB Henderson
Oct 24, 1998·Infection and Immunity·F GoulhenD Mayrand