Characterization of ovatoxin-h, a new ovatoxin analog, and evaluation of chromatographic columns for ovatoxin analysis and purification

Journal of Chromatography. a
Charline BrissardChristine Herrenknecht

Abstract

The presence of Ostreopsis cf. ovata on the Mediterranean coast represents a serious concern to human health due to production of toxins - putative palytoxin and ovatoxins (ovatoxin-a, -b, -c, -d, -e, -f and -g). However, purified ovatoxins are not widely available and their toxicities are still unknown. In the present study, we report on HR LC-MS/MS analysis of a French O. cf. ovata strain (IFR-OST-0.3V) collected at Villefranche-sur-Mer (France) during a bloom in 2011. Investigation of this strain of O. cf. ovata cultivated in our laboratory by ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS) confirmed the production of ovatoxins-a to -e and revealed the presence of a new ovatoxin analog, named ovatoxin-h. O. cf. ovata extracts were pre-purified by Sephadex LH-20 to obtain a concentrated fraction of ovatoxins (OVTXs). This method provided a recovery of about 85% of OVTXs and a cleanup efficiency of 93%. Different stationary phases were tested with this fraction of interest to elucidate the structure of the new OVTX congener and to obtain purified ovatoxins. Eight reversed phase sorbents were evaluated for their capacity to separate and purify ovatoxins. Among them Kinetex ...Continue Reading

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Citations

Feb 11, 2016·Marine Drugs·Marco PelinAurelia Tubaro
Dec 23, 2015·Journal of the Marine Biological Association of the United Kingdom·Elisa BerdaletHenrik Enevoldsen
Jun 28, 2016·Toxicon : Official Journal of the International Society on Toxinology·Valentina BrovedaniMarco Pelin
Feb 18, 2017·Marine Drugs·Penelope AjaniShauna A Murray
Aug 12, 2018·Toxins·Natalia VilariñoLuis M Botana
May 31, 2018·Marine Drugs·Federica FarabegoliAna García Cabado
Feb 15, 2020·Harmful Algae·Patricia A TesterElisa Berdalet
Feb 20, 2020·Marine Environmental Research·Stefano AccoroniPatricia M Glibert
Nov 14, 2017·Environmental Science & Technology·Luciana TartaglioneCarmela Dell'Aversano
May 7, 2020·Toxicon : Official Journal of the International Society on Toxinology·Lucía SoliñoPedro Reis Costa

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