Characterization of ovine TLR7 and TLR8 protein coding regions, detection of mutations and Maedi Visna virus infection

Veterinary Immunology and Immunopathology
Ivan MikulaIvan Mikula

Abstract

Toll-like receptors (TLRs) 2, 3, 4, 7, 8 and 9 play a crucial role in the recognition of viral entities and modulation of the innate immune system. This work presents sequence analysis of ovine TLR7 and TLR8 genes, depicts novel mutations and describes frequencies of mutations in Maedi Visna infected and healthy sheep. Totally 48 samples of the breed Tsigai were analyzed for the presence of mutations. Within 20 mutations, 14 were silent whereas 6 were missense. The frequencies of missense mutations in the Maedi Visna infected compared to non-infected sheep were: Lys115Glu (P-0.766, F-test), Asn117 (P-0.380) and Lys818Arg (P-0.739). These three mutations were localized in extra LRR (lucine rich repeat) region of TLR7, while mutation Ile73Leu (P-0.498) was located within LRR2 motif. Both mutations in TLR8, Asn165Lys (P-1.0) and Tyr349His (P-0.700), were present in extra LRR region. The secondary structure analysis of ovine TLR7 and TLR8 revealed conserved LRR motif structure, however with some irregularities compared to cattle and human. Transmembrane domains of TLR7 and TLR8 showed 100% homology between sheep and cattle wherein no mutations were found. In both TLRs TIR domains were highly conserved with occurrence of 4 silent mu...Continue Reading

References

Jul 1, 1994·The Journal of General Virology·B A BlacklawsI McConnell
Aug 29, 1996·Nature·J M Coffin
Oct 12, 2000·Journal of Virology·S RyanB Blacklaws
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·John C RassaSusan R Ross
Aug 7, 2002·Journal of Virology·Karen BiebackSibylle Schneider-Schaulies
Feb 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Richard MalleyDouglas T Golenbock
Aug 6, 2003·The Journal of Experimental Medicine·Jennifer LundAkiko Iwasaki
Oct 14, 2003·Trends in Immunology·Jessica K BellDavid M Segal
Dec 25, 2003·Journal of Virology·Dalia BurzynIsabel Piazzon
Feb 21, 2004·Science·Sandra S DieboldCaetano Reis e Sousa
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichi TabetaBruce Beutler
May 13, 2004·Clinical and Diagnostic Laboratory Immunology·Meriem Ben-AliKoussay Dellagi
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Jul 13, 2004·Acta Paediatrica·A DahlströmB Lundell
Apr 15, 2005·Molecular Immunology·Andrew G Bowie, Ismar R Haga
Jun 22, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Afig BerdeliHikmet Hakan Aydin
Jun 28, 2005·Nucleic Acids Research·J ChengP Baldi
Aug 18, 2005·The Journal of Biological Chemistry·Tadashi NishiyaAnthony L Defranco
Jun 28, 2007·European Journal of Immunology·Thomas R HawnAlan Aderem
Sep 18, 2007·Science·Shen-Ying ZhangJean-Laurent Casanova
Nov 16, 2007·International Journal of Immunogenetics·Y TabelS Mir
Dec 14, 2007·Molecular BioSystems·Alexandros G Georgakilas
Jan 8, 2008·The Journal of Infectious Diseases·Pierre-Yves BochudAlan Aderem
Jul 9, 2008·The Journal of Infectious Diseases·D-Y OhR R Schumann
Nov 21, 2008·Molecular Immunology·Jianzhong ZhuGeorge K Mutwiri

❮ Previous
Next ❯

Citations

Aug 26, 2014·Viruses·Nancy StonosNiel Karrow
Jul 24, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Soroush T SarvestaniMichael P Gantier
Nov 5, 2014·PloS One·Sakthivel DhanasekaranSubbiah Elankumaran
May 25, 2012·Tropical Animal Health and Production·Praveen Kumar DubeyRanjit Singh Kataria
Jan 28, 2021·International Journal of Molecular Sciences·Laura Kate GadanecVasso Apostolopoulos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.