Characterization of oxygen-tolerant Chinese hamster ovary cells. II. Energy metabolism and antioxidant status

Free Radical Biology & Medicine
P Van der ValkH Joenje

Abstract

Further characteristics of an oxygen-tolerant variant of Chinese hamster ovary cells (CHO-99) capable of stable proliferation at 99% O2/1% CO2, and O2 level that is lethal to the parental line (CHO-20), are described. Previous work has revealed that CHO-99 cells have 2- to 4-fold increased activities of superoxide dismutases, catalase and glutathione peroxidase, and substantially increased relative volumes of mitochondria and peroxisomes. To document possible additional mechanisms of O2 tolerance we compared CHO-20 cells growing at 20% O2 (normoxia) and CHO-99 cells at 99% O2 (normobaric hyperoxia). We show the following: (1) the estimated total (oxidative and glycolytic) ATP production in CHO-99 cells was 36% decreased. ATP production through oxidative phosphorylation was 52% lower in CHO-99 cells, while the relative contribution from glycolysis was increased from 6% to 30%. The ATP content was 29% lower in CHO-99 cells, the adenylate energy charge being also significantly decreased, indicating that energy production through oxidative phosphorylation is compromised in CHO-99 cells. Cyanide-resistant respiration was 4-fold higher in CHO-99 cells, probably reflecting, at least partly, the increased peroxisomal activity in these ...Continue Reading

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Citations

Jul 1, 1993·Biotechnology and Bioengineering·A A LinW M Miller
Jan 1, 1989·Journal of Cancer Research and Clinical Oncology·A H WanamartaH Joenje
Sep 15, 2012·Bioprocess and Biosystems Engineering·Atefeh GhorbaniaghdamMario Jolicoeur
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Oct 7, 2006·Free Radical Biology & Medicine·Li HuangDouglas Borchman
Feb 9, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S R RiddleC W White
Mar 10, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S AhmadC B Allen

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