Characterization of oxytocin receptor expression and distribution in the pregnant sheep uterus

Endocrinology
W X WuP W Nathanielsz

Abstract

At the end of pregnancy, the myometrium becomes extremely sensitive to oxytocin (OT) as result of a dramatic increase in the number of OT receptors (OTR), indicating an important role for OTR in the process of labor. There are no studies in sheep in which the physical properties and histological distribution of OTR are evaluated in relation to parturition. Also, no studies have been performed in any species to simultaneously examine the distribution of OTR at the messenger RNA (mRNA) as well as the protein levels in the same tissues and correlate those changes with the patterns of myometrial activity that occur at labor. In the present studies, we have used a polyclonal anti-OTR antibody and Western blot analysis to determine the apparent molecular mass of ovine OTR in late pregnant sheep myometrium and endometrium. We also examined the distribution of OTR mRNA and protein expression in the intact myometrium and endometrium and in individual cultured cells using in situ hybridization and immunocytochemistry. The expression of OTR and its mRNA has been correlated with the patterns of activity observed in the pregnant sheep myometrium. Western blot analysis of myometrial and endometrial extracts revealed a major form of OTR with ...Continue Reading

Citations

Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·J GutkowskaS M McCann
Jun 21, 2014·Reproduction in Domestic Animals = Zuchthygiene·A GramM P Kowalewski
Sep 11, 2004·Biochemical and Biophysical Research Communications·Masaki KawamataKatsuhiko Nishimori
Apr 23, 2017·Animal Reproduction Science·L Górriz-MartínM Heppelmann
Dec 15, 2016·Reproduction in Domestic Animals = Zuchthygiene·T M TamminenT Katila
Apr 1, 2014·Reproduction, Fertility, and Development·M de Ruijter-VillaniT A E Stout
Mar 20, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J G Ninomiya-AlarcónR Guevara-Guzmán
Apr 2, 2002·World Journal of Gastroenterology : WJG·Chuan-Yong LiuPaulus S Wang
Jan 25, 2000·American Journal of Physiology. Cell Physiology·W X WuP W Nathanielsz
Dec 7, 2006·American Journal of Physiology. Endocrinology and Metabolism·Masaki KawamataKatsuhiko Nishimori

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