Abstract
The level of beta-galactoside alpha2,6-sialyltransferase I (ST6Gal I) mRNA, encoded by the gene siat1, is increased in malignant tissues. Expression is regulated by different promoters - P1, P2 and P3 - generating three mRNA isoforms H, X and YZ. In cervical cancer tissue the mRNA isoform H, which results from P1 promoter activity, is increased. To study the regulation of P1 promoter, different constructs from P1 promoter were evaluated by luciferase assays in cervical and hepatic cell lines. Deletion of a fragment of 1048 bp (-89 to +24 bp) increased 5- and 3-fold the promoter activity in C33A and HepG2 cell lines, respectively. The minimal region with promoter activity was a 37 bp fragment in C33A cells. The activity of this region does not require the presence of an initiator sequence. In HepG2 cells the minimal promoter activity was detected in the 66 bp fragment. Sp1 (-32) mutation increased the promoter activity only in HepG2 cells. HNF1 mutation decreased promoter activity in HepG2 cell line but not in C33A cells. We identified a large region that plays a negative regulation role. The regulation of promoter activity is cell type specific. Our study provides new insights into the complex transcriptional regulation of sia...Continue Reading
References
Sep 1, 1990·Glycobiology·X WangJ T Lau
Mar 22, 1991·Science·H KatoR G Roeder
Aug 1, 1990·The Journal of Experimental Medicine·I StamenkovicS Funderud
Apr 7, 1989·Cell·S T Smale, D Baltimore
Dec 11, 1994·Nucleic Acids Research·A Shrivastava, K Calame
Mar 14, 1995·Biochimica Et Biophysica Acta·D A Aas-EngS Funderud
Apr 1, 1993·European Journal of Biochemistry·H C AasheimE B Smeland
May 17, 1996·The Journal of Biological Chemistry·B Manzano-WinklerA L Roy
Apr 1, 1996·Glycobiology·N W Lo, J T Lau
Oct 1, 1996·Molecular and Cellular Biology·E E FurlongF Martin
Nov 12, 1996·Biochemical and Biophysical Research Communications·N W Lo, J T Lau
Jan 19, 2000·Nucleic Acids Research·S KyoM Inoue
Jun 28, 2000·Nucleic Acids Research·K FujimotoM Inoue
Aug 30, 2000·International Journal of Cancer. Journal International Du Cancer·F Dall'OlioD Santini
Sep 1, 2001·Biochimie·A Harduin-LepersP Delannoy
Nov 20, 2001·Endocrinology·D LopezM P McLean
Jun 25, 2002·Gynecologic Oncology·Peng-Hui WangChiou-Chung Yuan
Apr 22, 2003·Genes & Development·Natalie Rezai-ZadehEdward Seto
Jun 12, 2003·Gynecologic Oncology·Peng Hui WangChiou Chung Yuan
Jul 25, 2003·Biochemical and Biophysical Research Communications·Liming XuAkiyoshi Taniguchi
May 10, 2005·The Journal of Biological Chemistry·Jung-Ahn LeeMan-Wook Hur
Jun 1, 1996·Clinical Molecular Pathology·F Dall'olio
Sep 12, 2006·The Journal of Biological Chemistry·Margaret Po-shan LukeYang Shi
Feb 18, 2011·Transcription·Zhiyong DengGuangchao Sui
Sep 13, 2011·Clinical Proteomics·Danni L Meany, Daniel W Chan
Citations
Jan 24, 2014·PloS One·Hongye MaLi Jia
Jan 13, 2015·Glycoconjugate Journal·Yan ZhangQun He
Apr 29, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jishun Lu, Jianguo Gu
Oct 16, 2019·Journal of Ovarian Research·Kaitlyn A DorsettSusan L Bellis
Jan 24, 2016·The Journal of Biological Chemistry·Jishun LuJianguo Gu
Dec 16, 2020·Glycobiology·Kaitlyn A DorsettSusan L Bellis