Characterization of p57, a Stage-Specific Antigen of Pneumocystis murina

The Journal of Infectious Diseases
Lisa R BishopJoseph A Kovacs

Abstract

Pneumocystis has a large multicopy gene family encoding proteins related to the major surface glycoprotein (Msg), whose functions are largely unknown. We expressed one such protein of Pneumocystis murina, p57, which is encoded by 3 highly conserved genes, and demonstrated by immunoblot that immunocompetent mice that were immunized with crude Pneumocystis antigens or that had cleared Pneumocystis infection developed antibodies to p57. Using hyperimmune anti-p57 serum combined with immunolabeling, we found that p57 was expressed by small trophic forms and intracystic bodies, whereas it was not expressed on larger trophic forms or externally by cysts. Expression of p57 and Msg by trophic forms was largely mutually exclusive. Treatment of infected animals with caspofungin inhibited cyst formation and markedly decreased p57 expression. While p57 expression was seen in immunocompetent mice infected with Pneumocystis, immunization with recombinant p57 did not result in altered cytokine expression by lymphocytes or in diminished infection in such mice. Thus, p57 appears to be a stage-specific antigen of Pneumocystis that is expressed on intracystic bodies and young trophic forms and may represent a mechanism to conserve resources in or...Continue Reading

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Citations

May 4, 2018·The Journal of Eukaryotic Microbiology·Melanie T CushionLouis M Weiss
Oct 18, 2018·Journal of Medical Microbiology·Theodore J KottomAndrew H Limper
Dec 7, 2018·PLoS Pathogens·Philippe M Hauser, Melanie T Cushion
Jun 21, 2020·The Journal of Infectious Diseases·Emanuel Schmid-SiegertPhilippe M Hauser

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