Characterization of Papaya ringspot virus isolates infecting transgenic papaya 'Huanong No.1' in South China

Scientific Reports
Zi-Lin WuHua-Ping Li

Abstract

In 2006, the release and cultivation of the genetically modified papaya cultivar 'Huanong No.1' successfully controlled the destructive papaya ringspot disease caused by Papaya ringspot virus (PRSV) in South China. However, some transgenic papaya plants from Guangdong and Hainan are found infected by PRSV. In this study, Field investigation was carried out and susceptible transgenic papaya samples were collected during 2012-2016. Twenty representative isolates were artificially inoculated into Cucurbita pepo and commercialised 'Huanong No.1' papaya, and results indicated that the plants showed obvious disease symptoms. Phylogenetic analysis of CP genes of 120 PRSV-infected isolates showed that PRSV can be divided into three groups. Isolates from Guangdong and Hainan belong to Group III, which is further divided into two subgroups. The isolates collected in this study have greatly diverged from the previously reported dominant strains Ys, Vb and Sm in South China, indicating that they belong to a new lineage. Further analysis showed a highly genetic differentiation between isolates, and 27.1% of the isolates were identified as recombinants on the basis of CP nucleotide sequences. These results indicate that the genetic variation...Continue Reading

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Citations

Aug 20, 2020·Plant Disease·Tathiana F Sá AntunesPatricia M B Fernandes
Jul 19, 2021·Horticulture Research·Maria Lobato-GómezPatricia Sarai Girón-Calva

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Datasets Mentioned

BETA
X9725.1
KF734962.1
EF183499.1

Methods Mentioned

BETA
transgenic

Software Mentioned

R Project for Statistical Computing
CLUSTALW
FEL
DnaSP
Arlequin
IFEL
Primer Premier
HyPhy
HyPhy package
GENECONV

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