PMID: 9419776Jul 1, 1994Paper

Characterization of paracellular permeability in cultured human cervical epithelium: regulation by extracellular adenosine triphosphate

Journal of the Society for Gynecologic Investigation
G I GorodeskiM F Romero

Abstract

The purpose of the present study was to compare the permeability and regulation of paracellular transport in human cervical cells with those in epithelial cells of other organs. Cervical cells (ECE16-1, Caski, and HT3) were grown on filters, and transepithelial electrical conductance (GT) and the permeability to pyranine (PPyr) were determined. Cervical cultures were characterized by high GT (83-125 mS.cm-2) and high PPyr (6.2-18 x 10(-6).sec-1). The GT was not significantly affected by cell density but was increased by 20% by lowering extracellular calcium to 0.45 mmol/L or less. The high values of GT and PPyr and the regulation by extracellular calcium indicate that all three cervical cell lines have "leaky" tight junctional complexes. Addition of extracellular adenosine triphosphate (ATP) at 50 mumol/L to the cervical cultures evoked a biphasic change in GT that was unique to the cervical cells: an initial increase, followed by a sustained decrease by 30% from baseline GT. The decrease of GT was associated with a decrease in PPyr by 17%, indicating that ATP had an effect on the tight junctional/paracellular permeability. The ATP effect was reversible either by washing or by chemical hydrolysis with ATPase. The non-cervical c...Continue Reading

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Citations

Nov 10, 2005·Menopause : the Journal of the North American Menopause Society·George I Gorodeski
Dec 18, 2001·American Journal of Physiology. Cell Physiology·George I Gorodeski
Mar 20, 2012·Expert Opinion on Drug Metabolism & Toxicology·Bruno SarmentoDomingos Ferreira
May 1, 1997·The American Journal of Physiology·G I Gorodeski, J Goldfarb
Nov 18, 2000·American Journal of Physiology. Cell Physiology·G I Gorodeski
Dec 18, 2001·American Journal of Physiology. Cell Physiology·George I Gorodeski
Dec 31, 1997·The American Journal of Physiology·G I GorodeskiE A Rorke
Oct 1, 1995·The American Journal of Physiology·G I GorodeskiU Hopfer
Oct 10, 2001·Fertility and Sterility·G I Gorodeski

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