Characterization of pbpA and pbp2 encoding penicillin-binding proteins located on the downstream of clavulanic acid gene cluster in Streptomyces clavuligerus

Biotechnology Letters
Kenji IshidaJae Kyung Sohng

Abstract

Two genes, pbpA (orf18) and pbp2 (orf19) located on the downstream of clavulanic acid (CA) gene cluster of Streptomyces clavuligerus were cloned into pET-28a(+), and confirmed to encode a family of high molecular-weight penicillin-binding proteins (PBPs). Both genes were amplified from genomic DNA by PCR and expressed in E. coli BL21 (DE3). Hydropathy plots of the proteins revealed a single stretch of hydrophobic amino acids indicating them to be transmembrane proteins. Pbp2 had lower affinity to penicillin G compared to PbpA, and was essential to the cell growth in contrast to PbpA.

References

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Citations

Jan 31, 2013·Applied Microbiology and Biotechnology·Aslıhan KurtGülay Özcengiz
May 1, 2008·Journal of Industrial Microbiology & Biotechnology·Paloma LirasIrene Santamarta
Jul 1, 2014·Journal of Biotechnology·Y Martínez-BurgoP Liras
May 14, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hiroshi Ogawara
Oct 30, 2014·The Journal of Antibiotics·Hiroshi Ogawara
Jun 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hiroshi Ogawara
Nov 9, 2012·Natural Product Reports·Refaat B HamedChristopher J Schofield
Sep 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hiroshi Ogawara

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