Characterization of pco-1, a newly identified gene which regulates purine catabolism in Neurospora

Current Genetics
T D Liu, G A Marzluf

Abstract

A new gene of Neurospora crassa, designated pco-1, was characterized and shown to regulate the expression of several genes which encode enzymes required for the catabolism of purines. Unlike the wild type, a pco-1 mutant created by repeat-induced point mutation cannot utilize purines as a nitrogen source. The PCO1 protein contains a Zn(II)2Cys6 binuclear cluster motif near its N-terminus, followed by a putative coiled-coil motif. A chemical crosslinking experiment demonstrated that PCO1 forms homodimers. PCO1 binds to CGG-N6-CCG elements located in the upstream promoter region of four genes encoding purine catabolic enzymes. Northern blot analysis demonstrated that a functional PCO1 protein is required for induction of xdh, which encodes xanthine dehydrogenase. Moreover, PCO1 was required for induction of three different purine catabolic enzymes. Two glutamine-rich domains occur in the C-terminal region of PCO1 and at least one of the glutamine-rich regions is required for PCO1 function, suggesting that they might play a role in transcriptional activation. The PCO1 protein does not interact with the global-acting NIT2 protein or the negative-acting NMR protein that functions in nitrogen catabolite repression. Induction of the x...Continue Reading

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Citations

Apr 9, 2013·Applied Microbiology and Biotechnology·Perng-Kuang Chang, Kenneth C Ehrlich
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hildur V ColotJay C Dunlap
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Feb 13, 2016·Microbiology and Molecular Biology Reviews : MMBR·Monika SchmollAlfredo Herrera-Estrella
Mar 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Lori B HubermanN Louise Glass

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