Characterization of Peptidyl-Prolyl Cis-Trans Isomerase- and Calmodulin-Binding Activity of a Cytosolic Arabidopsis thaliana Cyclophilin AtCyp19-3

PloS One
Gundeep KaurPrabhjeet Singh

Abstract

Cyclophilins, which bind to immunosuppressant cyclosporin A (CsA), are ubiquitous proteins and constitute a multigene family in higher organisms. Several members of this family are reported to catalyze cis-trans isomerisation of the peptidyl-prolyl bond, which is a rate limiting step in protein folding. The physiological role of these proteins in plants, with few exceptions, is still a matter of speculation. Although Arabidopsis genome is predicted to contain 35 cyclophilin genes, biochemical characterization, imperative for understanding their cellular function(s), has been carried only for few of the members. The present study reports the biochemical characterization of an Arabidopsis cyclophilin, AtCyp19-3, which demonstrated that this protein is enzymatically active and possesses peptidyl-prolyl cis-trans isomerase (PPIase) activity that is specifically inhibited by CsA with an inhibition constant (Ki) of 18.75 nM. The PPIase activity of AtCyp19-3 was also sensitive to Cu(2+), which covalently reacts with the sulfhydryl groups, implying redox regulation. Further, using calmodulin (CaM) gel overlay assays it was demonstrated that in vitro interaction of AtCyp19-3 with CaM is Ca(2+)-dependent, and CaM-binding domain is locali...Continue Reading

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Citations

Jan 6, 2017·Journal of Experimental Botany·Ziv SpiegelmanShmuel Wolf
May 8, 2019·International Journal of Molecular Sciences·Saleem AhmadYu Wang
May 6, 2017·Scientific Reports·Patrizia HanhartJulia Kehr
Sep 14, 2020·The Plant Journal : for Cell and Molecular Biology·Susan M MoengaDouglas R Cook
Nov 17, 2020·Frontiers in Plant Science·Harpreet SinghPrabhjeet Singh
May 1, 2021·Frontiers in Microbiology·Bishnu Maya BashyalRashmi Aggarwal

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Methods Mentioned

BETA
PCR
cleavage assay
X-ray
co-immunoprecipitation
immunoprecipitation
transfection

Software Mentioned

GraFit
TEMSP Predict
ESPript
UCFC Chimera
MATGAT
modeller
Treeview
TEMSP

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