Characterization of phenolic compounds in rooibos tea

Journal of Agricultural and Food Chemistry
Nicole Krafczyk, Marcus A Glomb

Abstract

Polyphenols present in rooibos, a popular herbal tea from Aspalathus linearis, were isolated in two steps. First, phenolic ingredients were separated by multilayer countercurrent chromatography (MLCCC). Preparative high-performance liquid chromatography (HPLC) was then applied to obtain pure flavonoids. The purity and identity of isolated compounds was confirmed by different NMR experiments, HPLC-diode array detector (DAD), or gas chromatography-mass spectrometry (GC-MS) analysis. This strategy proved to be valid to isolate material in up to gram quantities and to verify known and previously not published polyphenol structures. In addition the chemistry of dihydrochalcones and related intermediates was studied. The dihydrochalcone aspalathin was oxidized to the corresponding flavanone- C-glycosides (( R)/( S)-eriodictyol-6- C-beta- D-glucopyranoside and ( R)/( S)-eriodictyol-8- C-beta- D-glucopyranoside). Flavanone-6- C-beta- D-glucopyranosides were further degraded to flavones isoorientin and orientin.

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Citations

Apr 22, 2011·Analytical and Bioanalytical Chemistry·Ihsan IswaldiAlberto Fernández-Gutiérrez
May 11, 2012·Journal of Agricultural and Food Chemistry·Theres HeinrichMarcus A Glomb
Jul 28, 2013·Journal of Agricultural and Food Chemistry·Sarah HemmersbachHans-Ulrich Humpf
Dec 21, 2013·Journal of Natural Products·Ze HanJan H van der Westhuizen
Mar 13, 2010·Organic Letters·Akop YepremyanThomas G Minehan
Sep 13, 2011·Journal of Chromatography. a·Deirdre CabooterGert Desmet
Jan 8, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·R Beltrán-DebónJ Joven
Jul 16, 2015·Journal of Natural Products·J Brent FriesenGuido F Pauli
Jan 27, 2009·Clinics in Dermatology·Joerg Gruenwald
Jan 22, 2009·Molecular Nutrition & Food Research·Nicole KrafczykMarcus A Glomb
Jul 19, 2012·Journal of Separation Science·Theresa BeeldersAndré de Villiers
Dec 21, 2012·Journal of Separation Science·Yada Nolvachai, Philip J Marriott
Nov 23, 2013·Journal of Food Science·Makoto KondoTsuyoshi Watanabe
Feb 20, 2010·Journal of Separation Science·Fernanda das Neves Costa, Gilda Guimarães Leitão
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Fraser L Courts, Gary Williamson
May 3, 2014·Journal of Agricultural and Food Chemistry·Yi-Wen MaoMei-Hsien Lee
Dec 7, 2016·Electrophoresis·William J ArriesAndré de Villiers
Oct 23, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jin Dai, Russell J Mumper
Apr 6, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ondrej ZitkaRene Kizek
Aug 12, 2020·Alcoholism, Clinical and Experimental Research·David FisherSahar Abdul-Rasool
Dec 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Olawale R AjuwonGbenga A Adefolaju
May 27, 2009·Journal of Agricultural and Food Chemistry·Elizabeth JoubertMarena Manley
Jul 16, 2009·Journal of Agricultural and Food Chemistry·Nicole KrafczykMarcus A Glomb
Feb 10, 2009·Journal of Agricultural and Food Chemistry·Serena C MarksAlan Crozier

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