Characterization of phenotypically distinct B-cell subsets and receptor-stimulated mitogen-activated protein kinase activation in human cord blood B cells

Journal of Leukocyte Biology
Yun Jung HaJong Ran Lee

Abstract

Human cord blood (CB) is a valuable source of hematopoietic stem cells, but clinical reports have indicated slow recovery of B-cell development and function after CB transplantation. To investigate the basis of these B-cell defects in reconstitution, we characterized B cells purified from CB. We compared B-cell receptor activation and B-cell subsets in CB, bone marrow (BM), and peripheral blood (PB). We found that in CB B cells activation of extracellular signal-regulated kinase (ERK) and p38 following ligation of CD40 but not of the B-cell antigen receptor (BCR) was inefficient. The patterns of expression of CD5, CD34, and CD40 in the B-cell population of CB were similar to those in PB rather than in BM. The B cells in CB contained an increased proportion of B cells expressing a high level of CD24 and a low proportion of B cells expressing CD27, pointing to the presence of circulating CD24high immature transitional and CD27(-) naive B cells. CD40-mediated activation of ERK and p38 was also minimal in these B cells of CB. These findings may account for the functional defects of B cells in transplanted CB.

References

Jan 1, 1992·Advances in Immunology·J Banchereau, F Rousset
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·P RubinsteinC E Stevens
Aug 1, 1997·Seminars in Immunology·Y J Liu, J Banchereau
May 23, 1998·Annual Review of Immunology·I S Grewal, R A Flavell
Jul 29, 1998·Leukemia Research·H DimitriouM Kalmanti
Nov 6, 1998·The Journal of Experimental Medicine·S G TangyeJ E de Vries
Jun 17, 2000·Current Opinion in Immunology·K Hayakawa, R R Hardy
Mar 13, 2001·Annual Review of Immunology·R R Hardy, K Hayakawa
Mar 29, 2001·Experimental Hematology·A MorettaF Locatelli
Jun 18, 2003·Trends in Immunology·James B ChungJohn G Monroe
Feb 14, 2004·Immunological Reviews·Rita CarsettiHedda Wardmann
Nov 1, 2005·European Journal of Immunology·Stefan Wirths, Antonio Lanzavecchia
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amanda K CussStuart G Tangye
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Angela MalaspinaAnthony S Fauci

❮ Previous
Next ❯

Citations

Oct 24, 2012·The Journal of Experimental Medicine·Marc SeifertRalf Küppers
Aug 31, 2013·Annual Review of Pathology·Suping Zhang, Thomas J Kipps
Feb 16, 2019·American Journal of Reproductive Immunology : AJRI·Yaozhu LengNardhy Gomez-Lopez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.