PMID: 9449782Jan 1, 1997Paper

Characterization of phytase produced by Aspergillus niger

Folia Microbiologica
J DvorákováJ Kopecký

Abstract

The extracellular activity of Aspergillus niger phytase at the end of the growth phase was 132 nkat/mL in a laboratory bioreactor. The purified enzyme has molar mass approximately 100 kDa, pH optimum at 5.0, temperature optimum at 55 degrees C and high pH and temperature stability. The Km for dodecasodium phytate, calcium phytate and 4-nitrophenyl phosphate are 0.44, 0.45 and 1.38 mmol/L, respectively. The enzyme is noncompetively inhibited by inorganic monophosphate (Ki = 2.85 mmol/L) and by Cu2+, Zn2+, Hg2+, Sn2+, Cd2+ ions and strongly by F- ones; it is activated by Ca2+, Mg2+ and Mn2+ ions. The substrate specificity of phytase is broad with the highest affinity to calcium phytate.

Citations

Sep 7, 2000·Biochemical and Biophysical Research Communications·E J MullaneyA H Ullah
Mar 29, 2001·Folia Microbiologica·Y H TsengS M Tseng
Nov 20, 1998·Folia Microbiologica·J Dvoráková
Nov 28, 2007·Marine Biotechnology·Xiaoyu LiNalini Yasoda Hirimuthugoda
May 16, 2008·Folia Microbiologica·K PavlovaZ Tankova
May 4, 2011·The Journal of Microbiology·Patcharaporn PandeeVithaya Meevootisom
Nov 25, 2003·Biochemical and Biophysical Research Communications·Edward J Mullaney, Abul H J Ullah
Oct 3, 2002·Biochemical and Biophysical Research Communications·Edward J MullaneyAbul H J Ullah
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May 21, 2014·Preparative Biochemistry & Biotechnology·Seung Ho LeePeter C W Lee
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Apr 3, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xiao-Yun WangHai-Meng Zhou
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Apr 25, 2006·FEMS Microbiology Letters·Xiao-Ling ZhouZheng-Xiang Wang
Mar 29, 2001·Folia Microbiologica·J DvorákováV Kren

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