PMID: 1213683Dec 1, 1975Paper

Characterization of pig pancreatic kallikreins A and B

Hoppe-Seyler's Zeitschrift für physiologische Chemie
F FiedlerE Werle

Abstract

Pig pancreatic kallikreins A and B are both composed of the same 229 amino acids, a figure resembling the number of amino acid residues found in other serine proteinases of pancreas. Both forms of the enzyme contain N-terminal isoleucine and alanine and C-terminal leucine/serine (about half a mol each per mol kallikrein) and proline. Values for the glucosamine content of the kallikreins obtained on the amino acid analyzer after hydrolysis with p-toluenesulfonic acid, a procedure also used for the determination of amide ammonia, agreed with those determined by a gas-chromatographic method. Neuraminidasetreated kallikrein B differs from the A form only in containing roughly double the amount (on the average a total of 11.5 vs. 5.6% by weight) of carbohydrate (glucosamine, mannose, galactose, and fucose) and possibly by a higher content (20 vs. 17 residues) of amide ammonia. From the composition, molecular weights of 26800 and 28600 are calculated for sialic-acid-free kallikreins A and B, respectively, and of 25300 for the protein part of kallikrein. The molar absorbance of both forms of the enzyme has been determined as (50.6 +/- 1.3) X 10(3)M-1 cm-1 at 280 nm. A comparison of kallikreins A and B with kallikreins d1 and d2 descri...Continue Reading

Citations

Jun 1, 1992·Journal of Molecular Recognition : JMR·N P Burton, C R Lowe
Sep 1, 1980·Agents and Actions·R GeigerB Förg-Brey
Jan 1, 1979·Toxicon : Official Journal of the International Society on Toxinology·C C ViljoenD P Botes
Feb 1, 1986·Archives of Biochemistry and Biophysics·K K MurthyA G Scicli
Apr 28, 1989·Biochimica Et Biophysica Acta·M KouyoumdjianJ L Prado
Dec 1, 1977·Analytical Biochemistry·D M Kirschenbaum
Mar 1, 1981·Hoppe-Seyler's Zeitschrift für physiologische Chemie·M S Araujo-VielE S Prado
Sep 1, 1981·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F Fiedler, C Hirschauer
Jan 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·M Amouric, C Figarella
Apr 11, 1986·Journal of Chromatography·T HondaK Morihara

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