Characterization of Polo-like kinase-1 in rat oocytes and early embryos implies its functional roles in the regulation of meiotic maturation, fertilization, and cleavage

Molecular Reproduction and Development
Heng-Yu FanQing-Yuan Sun

Abstract

Polo-like kinase 1 (Plk1) is a family of serine/threonine protein kinases that play important regulatory roles during mitotic cell cycle progression. In this study, Plk1 expression, subcellular localization, and possible functions during rat oocyte meiotic maturation, fertilization, and embryonic cleavages were studied by using RT-PCR, Western blot, confocal microscopy, drug-treatments, and antibody microinjection. Both the mRNA and protein of this kinase were detected in rat maturing oocytes and developing embryos. Confocal microscopy revealed that Plk1 distributed abundantly in the nucleus at the germinal vesicle (GV) stage, was associated with spindle poles during the formation of M-phase spindle, and was translocated to the spindle mid-zone at anaphase. In fertilized eggs, Plk1 was strongly stained in the cytoplasm between the apposing male and female pronuclei, from where microtubules radiated. Throughout cytokinesis, Plk1 was localized to the division plane, both during oocyte meiosis and embryonic mitosis. The specific subcellular distribution of Plk1 was distorted after disrupting the M-phase spindle, while additional aggregation dots could be induced in the cytoplasm by taxol, suggesting its intimate association with a...Continue Reading

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Citations

Apr 26, 2008·Histochemistry and Cell Biology·Heide Schatten
Oct 5, 2010·Zygote : the Biology of Gametes and Early Embryos·Ke WangSheng-Sheng Lu
Feb 18, 2004·The Journal of Reproduction and Development·Qing-Yuan Sun, Takashi Nagai
Feb 14, 2008·Molecular Reproduction and Development·Shao-Chen SunQing-Yuan Sun
Sep 10, 2005·Mitochondrion·Heide SchattenQing-Yuan Sun
Sep 27, 2015·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yin-Li ZhangHeng-Yu Fan
Jul 16, 2015·Zygote : the Biology of Gametes and Early Embryos·Vladimir BaranPetr Solc
Apr 24, 2007·Developmental Biology·Lynda K McGinnisWilliam H Kinsey
Feb 13, 2018·Reproduction in Domestic Animals = Zuchthygiene·S HuC Zhu
Jul 28, 2004·Molecular Reproduction and Development·Martin AngerRichard M Schultz
Sep 30, 2005·Cytogenetic and Genome Research·J B Mailhes, F Marchetti
May 8, 2013·Molecular Reproduction and Development·V BaranP Sutovsky
Aug 31, 2017·Reproductive Biology and Endocrinology : RB&E·Zixiao ZhangShiqiang Ju

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