Characterization of poly-gamma-glutamate hydrolase encoded by a bacteriophage genome: possible role in phage infection of Bacillus subtilis encapsulated with poly-gamma-glutamate

Applied and Environmental Microbiology
Keitarou Kimura, Yoshifumi Itoh

Abstract

Some Bacillus subtilis strains, including natto (fermented soybeans) starter strains, produce a capsular polypeptide of glutamate with a gamma-linkage, called poly-gamma-glutamate (gamma-PGA). We identified and purified a monomeric 25-kDa degradation enzyme for gamma-PGA (designated gamma-PGA hydrolase, PghP) from bacteriophage PhiNIT1 in B. subtilis host cells. The monomeric PghP internally hydrolyzed gamma-PGA to oligopeptides, which were then specifically converted to tri-, tetra-, and penta-gamma-glutamates. Monoiodoacetate and EDTA both inhibited the PghP activity, but Zn(2+) or Mn(2+) ions fully restored the enzyme activity inhibited by the chelator, suggesting that a cysteine residue(s) and these metal ions participate in the catalytic mechanism of the enzyme. The corresponding pghP gene was cloned and sequenced from the phage genome. The deduced PghP sequence (208 amino acids) with a calculated M(r) of 22,939 was not significantly similar to any known enzyme. Thus, PghP is a novel gamma-glutamyl hydrolase. Whereas phage PhiNIT1 proliferated in B. subtilis cells encapsulated with gamma-PGA, phage BS5 lacking PghP did not survive well on such cells. Moreover, all nine phages that contaminated natto during fermentation pro...Continue Reading

References

Aug 1, 1976·Virology·H P Bernheimer, J G Tiraby
Oct 1, 1979·Canadian Journal of Microbiology·M L SaxelinV Sundman
Feb 1, 1979·Infection and Immunity·B J Wilkinson, K M Holmes
Feb 1, 1978·Applied and Environmental Microbiology·S BurtD T Jones
Jan 1, 1989·Journal of Clinical Microbiology·V J BenedíJ M Tomás
Jan 1, 1988·Medical Microbiology and Immunology·Yoshifumi OhshimaG Pulverer
Aug 1, 1982·Journal of Virology·B KwiatkowskiS Stirm
Oct 1, 1995·Research in Microbiology·H W AckermannM X Yu
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·R YaoJ Galivan
Jun 10, 1998·Analytical Biochemistry·E Valero, F Garcia-Carmona
Mar 31, 2000·Pharmacology & Therapeutics·J GalivanD Yin
Oct 19, 2001·European Journal of Biochemistry·Makoto AshiuchiHaruo Misono
Dec 26, 2001·Journal of Bacteriology·Yuji UrushibataYasutaka Tahara
Sep 1, 1954·Journal of Bacteriology·C B THORNER D HOUSEWRIGHT

Citations

Jan 31, 2013·Applied Microbiology and Biotechnology·Wei ZengZhiqun Liang
Sep 8, 2010·Journal of Industrial Microbiology & Biotechnology·Eun Ju KimYoung Nam Lee
Apr 6, 2004·FEMS Microbiology Letters·Ian W SutherlandKaren Tait
Jan 31, 2012·Journal of Agricultural and Food Chemistry·Qun Wu, Yan Xu
Sep 3, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Zui FujimotoKeitarou Kimura
Jul 19, 2011·Applied and Environmental Microbiology·Yuji KuboKeitarou Kimura
Oct 4, 2011·Applied and Environmental Microbiology·Thi-Huyen DoKeitarou Kimura
May 8, 2009·Bioscience, Biotechnology, and Biochemistry·Keitarou KimuraYoshifumi Itoh
Aug 16, 2013·International Journal of Nanomedicine·Ramanathan K KasimanickamJohn P Kastelic
Aug 21, 2013·The Journal of Chemical Physics·Brian J NabloJohn J Kasianowicz
Apr 9, 2013·Bioscience, Biotechnology, and Biochemistry·Shigeki KadaKen-ichi Yoshida
Jan 16, 2016·Applied Microbiology and Biotechnology·Diana P PiresJoana Azeredo
May 13, 2006·Molecular Microbiology·Thomas Candela, Agnès Fouet
Aug 15, 2015·BioMed Research International·Anna KrasowskaMarcin Łukaszewicz
Jan 24, 2009·Trends in Microbiology·Rodney M Donlan
Sep 6, 2006·Journal of Bioscience and Bioengineering·Makoto AshiuchiHaruo Misono
Feb 21, 2018·Microbiology·Tatsuya FukushimaJunichi Sekiguchi
Apr 11, 2014·American Journal of Rhinology & Allergy·Amanda DrillingPeter-John Wormald
Nov 3, 2018·The FEBS Journal·Sneha RamaswamyAnnalisa Pastore
Nov 28, 2018·Biomolecular Concepts·Ekachai ChukeatiroteJuhee Ahn
Aug 15, 2009·Chembiochem : a European Journal of Chemical Biology·Justin J Richards, Christian Melander
Mar 17, 2007·Critical Reviews in Biotechnology·Joerg M Buescher, Argyrios Margaritis
Jan 22, 2013·Biotechnology and Bioengineering·Viola ScoffoneCinzia Calvio
Mar 25, 2017·Applied Microbiology and Biotechnology·Agnieszka LatkaZuzanna Drulis-Kawa
Jan 31, 2020·Frontiers in Microbiology·Leandra E KnechtLars Fieseler
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Hong-Fei LiHua Tang
May 31, 2019·Biofouling·Legesse Geredew KifelewPeter Speck
Jul 6, 2019·Journal of Bioscience and Bioengineering·Le Thi Thu HongKeitarou Kimura

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