PMID: 8969465Jun 1, 1996Paper

Characterization of polydnavirus-encoded mRNA in parasitized armyworm larvae

Insect Biochemistry and Molecular Biology
A YamanakaH Watanabe

Abstract

We have isolated five cDNA clones encoding Cotesia kariyai polydnavirus (CkPDV) mRNAs transcribed in parasitized host larvae of Pseudaletia separata. One of the cDNAs encoding the longest 2.0 kb CkPDV mRNA was sequenced and characterized. Southern hybridization analyses using the cloned cDNA as a probe showed that CkPDV mRNA was homologous to one of CkPDV DNA segments, 5.6 kbp DNA segment A. The 2.0-kb mRNA was demonstrated as being expressed in the parasitized host larvae by Northern-blot analyses. When specific host tissues were examined, the 2.0-kb mRNA was detected mainly in haemocytes. This RNA increased in relative abundance after 2 and 4 h post-parasitization when the immune response of host haemocytes appeared compromised.

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Citations

May 29, 2003·Journal of Insect Physiology·K TanakaY Hayakawa
May 25, 2002·European Journal of Biochemistry·Kohjiro TanakaYoichi Hayakawa
Jun 2, 2005·In Vitro Cellular & Developmental Biology. Animal·Y P ChenD E Lynn
Nov 13, 2004·Trends in Microbiology·Richard V GlatzOtto Schmidt
Mar 7, 2009·The Journal of General Virology·Jae Young ChoiYeon Ho Je
Mar 17, 2004·DNA Sequence : the Journal of DNA Sequencing and Mapping·Cong Zhang, Chen-Zhu Wang
Jun 22, 2000·The Journal of General Virology·C BéliveauG Bellemare
Nov 29, 2021·Current Opinion in Insect Science·Erik H Poelman, Antonino Cusumano

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