Characterization of polymorphisms and isoforms of the Clostridium perfringens phospholipase C gene (plc) reveals high genetic diversity

Veterinary Microbiology
F F SiqueiraEvanguedes Kalapothakis

Abstract

Clostridium perfringens phospholipase C (Cp-PLC), also called alpha-toxin, is encoded by the plc gene and has been implicated in several diseases; however, only a few studies have described polymorphisms in this gene. The aim of this study was to analyze polymorphisms in the Cp-PLC nucleotide and amino acid sequences obtained from isolates from different regions and to compare them to Clostridium phospholipase C sequences deposited in the NCBI database. Environmental samples (sediment, poultry feed, sawdust) and stool samples (from poultry, bovine, swine, horse, caprine, bird, dog, rabbit, toucan) were collected from healthy and sick animals. A total of 73 isolates were analyzed with the majority of samples belonging to the toxin type A subtype and possessing the gene encoding for the beta-2 toxin. Comparison of plc gene sequences from respective isolates revealed a high genetic diversity in the nucleotide sequences of mature Cp-PLC. Sequence comparisons identified 30 amino acid substitutions and 34 isoforms including some isoforms with substitutions in amino acids critical to toxin function. Comparison of sequences obtained in this study to Cp-PLC sequences obtained from the NCBI database resulted in the identification of 11 c...Continue Reading

References

Jan 13, 1998·Gene·M GibertC Jolivet-Renaud
Aug 12, 1998·Nature Structural Biology·C E NaylorA K Basak
Jan 19, 1999·Annual Review of Microbiology·J I Rood
Apr 22, 1999·Trends in Microbiology·L PetitM R Popoff
Mar 9, 2004·Journal of Clinical Microbiology·Scott A SheedyRobert J Moore
Aug 5, 2006·Anaerobe·R W TitballA K Basak
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Mar 8, 2013·Anaerobe·Rodrigo Otávio Silveira SilvaFrancisco Carlos Faria Lobato
Mar 25, 2016·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Rodrigo O S SilvaFrancisco C F Lobato
Jul 1, 2014·Anaerobe·Rodrigo Otávio Silveira SilvaFrancisco Carlos Faria Lobato
May 3, 2019·Microbiology and Immunology·Asuka MakinoEmiko Isogai

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