PMID: 6200441Apr 1, 1984

Characterization of polysaccharide antigens of Streptococcus mutans B13 grown under various conditions

Infection and Immunity
R LinzerK W Knox

Abstract

Bacteria may respond to changes in their environment by varying the synthesis of surface components. This study examined the effects of various culture conditions on two wall polysaccharides of Streptococcus mutans B13 (serotype d): serotype d antigen, a galactose-glucose polymer, and RGP antigen, a rhamnose-glucose polymer. Cells were grown in a chemostat at various dilution rates (D) and pH values, including D = 0.05, 0.1, and 0.5 h-1 at pH 6.0 and D = 0.1 h-1 at pH 5.5, 6.0, 6.5, and 7.5. The cells were examined for protein and carbohydrate content by colorimetric assays and gas-liquid chromatography. Rantz-Randall extracts (120 degrees C, 30 min) and M-1 N-acetylmuramidase digests were prepared and examined for the presence of specific antigens by agar gel diffusion and quantitative precipitation assays. Cell preparations did not vary significantly with respect to total protein or carbohydrate content; however, cells grown at D = 0.1 h-1 and pH 7.5 had a significantly higher rhamnose content than did the other preparations. Rapidly growing cultures appeared to be more resistant to M-1 N-acetylmuramidase digestion than did slower-growing cultures. Agar gel diffusion studies demonstrated that both serotype d and RGP antigens ...Continue Reading

Citations

Oct 16, 2014·Journal of Oral Microbiology·Zachary D MoyeRobert A Burne

Related Concepts

Endo-N-acetylmuramidase
Antigenic Specificity
Antigens, Bacterial
Bacterial Proteins
Sugars
Cell Wall
Exoglycosidases
Hydrogen-Ion Concentration
Immunosorbent Techniques
Polysaccharides, Bacterial

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