PMID: 7031232Aug 1, 1981Paper

Characterization of populations of promastigotes of Leishmania donovani

The Journal of protozoology
T I Doran, R Herman

Abstract

Promastigotes of Leishmania donovani cultured for either 3 or 10 days in vitro and inoculated intracardially into golden hamsters with an equal number of organisms from either population showed a 7-fold difference in infectivity when compared at both 10 and 16 days post-infection. Reproducible histochemical staining for the promastigote enzymes glucose-6-phosphate dehydrogenase (G6PDH) and peptidase after polyacrylamide gel electrophoresis showed two isoelectric variants of G6PDH (Bands 1 and 2) that displayed a 45% decrease (Band 1) and a 60% increase (Band 2) in total activity when 3- and 10-day-old promastigotes were compared. Peptidase activity, present in a single band, increased 7-fold in 10-day-old promastigotes. A decrease in the lectin-induced agglutination of promastigotes by castor bean agglutinin (RCA60), specific for D-galactose and N-acetyl-D-galactosamine, was seen when 3- and 10-day-old promastigotes are compared. Antisera raised against sonicated 10-day-old promastigotes showed a unique precipitin band between the antiserum and sonicated 10-day-old promastigotes not found between the antiserum and sonicated 3-day-old promastigotes.

References

Dec 31, 1979·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R LainsonJ J Shaw
Dec 1, 1979·Experimental Parasitology·U BachrachC L Greenblatt
Apr 1, 1977·Experimental Parasitology·D M Dwyer
Jan 1, 1977·Transactions of the Royal Society of Tropical Medicine and Hygiene·R F Steiger, S R Meshnick
Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·M Al-Taqi, D A Evans
Oct 1, 1978·Proceedings of the Society for Experimental Biology and Medicine·L H Semprevivo
Sep 1, 1975·Annals of Tropical Medicine and Parasitology·N M Mattock
Oct 1, 1974·The Journal of protozoology·M J Alves, W Colli
May 1, 1974·The Journal of protozoology·A J MukkadaC Neu
Nov 1, 1974·Nature·A R Stevens, A E Kaufman
Nov 1, 1968·The Journal of protozoology·N Entner
Feb 1, 1971·Experimental Parasitology·D K Ghosh, A G Datta
Feb 1, 1966·Experimental Parasitology·L A Stauber
Feb 1, 1954·The Biochemical Journal·S CROWTHERL P JOYNER
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Jan 1, 1964·Advances in Parasitology·S ADLER
Nov 1, 1949·Transactions of the Royal Society of Tropical Medicine and Hygiene·J D FULTON, L P JOYNER

❮ Previous
Next ❯

Citations

Sep 1, 1989·International Journal for Parasitology·D J MallinsonG H Coombs
Sep 1, 1989·International Journal for Parasitology·D J Mallinson, G H Coombs
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·E A MohammedK W Pondman
Aug 1, 1985·Molecular and Biochemical Parasitology·T J Nolan, J P Farrell
Jan 15, 1988·Molecular and Biochemical Parasitology·J A Kink, K P Chang
Oct 27, 2009·Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases·Bao-Qian JingJie Zhang
Apr 1, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ramu SivakumarSarman Singh
Jun 1, 1986·Experimental Parasitology·K NagakuraT Nakae
Feb 1, 1985·The Journal of protozoology·T J Nolan, R Herman
Jan 18, 2006·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·T J Bogdan, P G Judge
Mar 4, 2003·Revista Da Sociedade Brasileira De Medicina Tropical·Roberta Dias Rodrigues RochaOlindo Assis Martins-Filho
Mar 30, 1984·Science·D L Sacks, P V Perkins

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.