PMID: 376310Apr 1, 1979Paper

Characterization of porins from the outer membrane of Salmonella typhimurium. 1. Chemical analysis

European Journal of Biochemistry
H TokunagaT Nakae

Abstract

The three species of channel-forming outer membrane proteins, porins, have been purified to homogeneity from mutant strains of Salmonella typhimurium which produce single species of porin. Purification was by stepwise solubilization with dodecylsulfate or guanidine thiocyanate, gel filtration, and preparative gel electrophoresis. Amino acid compositions and tryptic peptide maps of the three species of porins showed close resemblance, but at the same time clear differences among them. The number of amino acid residues in the porins purified from the strains SH5551, SH6377 and SH6017 were 361, 354 and 345, and their calculated molecular weights 39800, 39300 and 38000, respectively. Amino-terminal and carboxyl-terminal amino acids in all three species of porins appeared to be alanine and phenylalanine, respectively. Neither half-cystine nor hexosamine was found in these preparation of porins. The isoelectric points of porins from the strains SH5551, SH6377 and SH6017, determined by isoelectric focusing, showed slight differences from each other. These results, and the genetic experiments from another laboratory, suggest that the three species of porins in Salmonella typhimurium are distinct polypeptides, probably coded for by dist...Continue Reading

References

Aug 1, 1977·Journal of Bacteriology·J F Lutkenhaus
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Citations

Jan 1, 1985·Pharmacology & Therapeutics·H Nikaido
Aug 26, 1998·Scandinavian Journal of Immunology·S Gupta
Jan 1, 1990·Microbiology and Immunology·K Matsui, T Arai
Sep 10, 1999·Microbiology and Immunology·M SinghH Vohra
Nov 1, 1982·Antimicrobial Agents and Chemotherapy·Y KobayashiT Nakae
Mar 1, 1988·Antimicrobial Agents and Chemotherapy·J Ishii, T Nakae
Apr 1, 1994·Immunological Investigations·M KawaharaG J Domingue
Oct 1, 1995·Kidney International·W WelteK Diederichs
Dec 1, 1994·Immunobiology·M KawaharaG J Domingue
Jan 1, 1989·Microbiology and Immunology·K Matsui, T Arai
Jan 1, 1985·CRC Critical Reviews in Biochemistry·R Benz
Feb 1, 1992·FEMS Microbiology Immunology·S Muthukkumar, V R Muthukkaruppan
Jan 1, 1986·Critical Reviews in Microbiology·T Nakae
Jun 20, 2002·Protein Expression and Purification·Steven ArcidiaconoCharlene M Mello
Sep 1, 1983·Microbiological Reviews·K E Sanderson, J R Roth
Jul 1, 1993·Infection and Immunity·S Muthukkumar, V R Muthukkaruppan
Mar 1, 1986·Journal of Bacteriology·R BenzG H Vos-Scheperkeuter

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