Characterization of Precision-cut Rat Lung Slices in a Biphasic Gas/Liquid Exposure System: Effect of O(2)

Toxicology in Vitro : an International Journal Published in Association with BIBRA
C MonteilJ P Fillastre

Abstract

Influence of oxygen on lung cell differentiation has been studied in precision-cut rat lung slice cultures. Rat lung slices were positioned on rolling inserts placed into vials with opened caps to allow free access to the gaseous phase. This system was placed into a continuous-flow rotating chamber with controlled pO(2), pCO(2) and hygrometry. Slices were cultured in a serum-free medium up to 3 days under an atmosphere of 21 or 70% oxygen. Cellular antioxidant markers demonstrated an oxygen concentration-dependent response. Slices cultured with 70% oxygen exhibited the highest specific activity of catalase, NADPH cytochrome c reductase and gamma-glutamyl transpeptidase (GGT) as well as the highest levels of intracellular glutathione after 48 or 72 hours of incubation. Moreover, hyperoxic exposure altered the expression of lung manganese-containing superoxide dismutase mRNA. Hyperoxia had little or no effect on intracellular ATP levels, which remained high in lung slices compared with freshly isolated tissue. The study of the pulmonary specific functions allowed to confirm maintenance of the in vitro cellular differentiation of lung slices incubated with 21% oxygen: (i) polyamine transport is preserved and exhibited kinetic prop...Continue Reading

References

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Citations

Oct 4, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Jean-Paul MorinChristelle Monteil
Oct 23, 2018·Drug Metabolism Letters·Yildiz YilmazGian Camenisch

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