PMID: 8605230Feb 9, 1996Paper

Characterization of prenyl protein transferase enzymes in a human keratinocyte cell line

Biochimica Et Biophysica Acta
E E MacNulty, N S Ryder

Abstract

Prenylation is a post-translational modification of proteins that involves the attachment of an isoprenoid group derived from mevalonic acid, either 15-carbon farnesyl or 20-carbon geranylgeranyl, to a specific carboxy-terminal domain of acceptor proteins. Three prenyl transferase enzymes have been identified so far. In this paper we report the presence of two prenyl transferases in the HaCaT human keratinocyte cell line. Chromatography of a cytosolic extract from these cells resolved a farnesyl protein transferase (FPT) and geranylgeranyl protein transferase-I (GGPT-I) whose activities were measured using a novel peptide-based assay. Both enzymes were inhibited dose dependently by zaragozic acids A and C. Zaragozic acid C was more active towards the FPT than GGPT-I while zaragozic acid A inhibited both enzymes with similar potency. Incubation of HaCaT cell homogenates with [3H] prenyl precursors resulted in the labelling of a number of proteins which was increased when the cells were pretreated with an inhibitor of hydroxymethylglutaryl CoA reductase. Given the role of prenylated proteins in proliferative and inflammatory processes, our finding that prenyl transferases capable of prenylating endogenous substrates are also pres...Continue Reading

References

Jan 1, 1992·Annual Review of Genetics·W R Schafer, J Rine
Dec 1, 1992·Current Opinion in Cell Biology·A D Cox, C J Der
Jan 1, 1992·Annual Review of Biochemistry·S Clarke
Sep 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·M S BrownJ C Marsters
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K KatoC J Der
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R Khosravi-FarC J Der
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·W J ChenM S Brown
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P J CaseyJ F Moomaw
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·W W EpsteinH C Rilling
Nov 14, 1991·Biochemical and Biophysical Research Communications·T S VincentE Merler
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y ReissJ L Goldstein
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A A FinegoldF Tamanoi
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K YokoyamaM H Gelb
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H K YamaneJ A Glomset
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyP C Sternweis
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V ManneH F Kung
Jan 19, 1990·Science·C C FarnsworthJ A Glomset
Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Jan 1, 1983·Methods in Enzymology·R Roskoski
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J D BergstromO D Hensens
Jan 1, 1993·The Journal of Clinical Investigation·M P O'DonnellW F Keane
Apr 1, 1993·The Journal of Antibiotics·K HasumiA Endo
Nov 1, 1993·Current Biology : CB·J F Hancock
Nov 1, 1990·The Journal of Investigative Dermatology·G J FisherJ J Voorhees
Nov 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ryang Hwa LeeDarwin J Prockop

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Citations

Jul 18, 2006·Analytical Biochemistry·Fabio Luiz D'AlexandriAlejandro Miguel Katzin

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