PMID: 8939814Dec 1, 1996Paper

Characterization of primary lesions caused by the plastome mutator of Oenothera

Current Genetics
T L ChangB B Sears

Abstract

Oenothera plants homozygous for a recessive allele at the plastome mutator (pm) locus show non-Mendelian mutation frequencies that are 1000-fold higher than spontaneous levels. Characterization of RFLP sites in a collection of mutants indicates that insertion-deletion hot spots in the pm lines are defined by tandem direct repeats, implicating replication slippage or misalignment during recombination. Several sites known to contain very short direct repeats were examined, and all were found to have been targeted in one or more plants of the mutant collection. To determine if replication slippage was occurring, two oligo-A stretches in non-coding DNA were examined, and 3 of 12 plants were found to have an additional adenine in a 13-base track. To search for other mutations that would not be visible as restriction fragment length polymorphisms, PCR-amplification products of the psbB gene were digested with a restriction endonuclease, denatured, and examined for single-strand conformational polymorphisms. Among 21 mutants, one 4-bp insertion and one point mutation were identified in psbB. The discovery that the plastome mutator can cause base substitutions as well as repeat-mediated insertions and deletions points to a likely defec...Continue Reading

Citations

Oct 6, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Lev A Elkonin
Feb 9, 2010·Molecular Genetics and Genomics : MGG·Rudolf Hagemann
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexandre MaréchalNormand Brisson
Jan 1, 2002·The Arabidopsis Book·Steven Rodermel
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Robert K JansenJeffrey L Boore
Feb 1, 2007·Plant, Cell & Environment·Fei YuSteven Rodermel
Feb 26, 2010·The New Phytologist·Alexandre Maréchal, Normand Brisson
Jul 22, 2017·The New Phytologist·Thomas W A BraukmannJohn V Freudenstein
Sep 27, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M GuhaMajumdarB B Sears
Mar 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Johanna SobanskiStephan Greiner

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