Characterization of prostatic neuroendocrine cell line established from neuroendocrine carcinoma of transgenic mouse allograft model

The Prostate
Kohsuke UchidaTaiji Tsukamoto

Abstract

The role of neuroendocrine (NE) cells in prostate cancer remains unclear. A useful model is necessary to study the biology of NE cells. We herein describe the establishment and characterization of an immortalized cell line from an NE-10 allograft of murine prostatic NE carcinoma. A novel cell line, designated NE-CS, was developed from an NE-10 allograft that was established from the ventral prostate of the LPB-T-antigen (Tag) transgenic mouse, line 10 (12T-10). We investigated the growth, karyotype, electron microscopic findings, expression of Tag and androgen receptor (AR), and tumorigenesis of the cells in athymic mice. The immortal cell line NE-CS was maintained in vitro for more than 2 years. The NE-CS cells had dendritic-like extensions with dense core granules in the cytoplasm and produced serotonin and somatostatin in conditioned medium. The cells expressed neither Tag nor AR. They showed androgen-independent growth in vitro and a hypotetraploid karyotype similar to the original NE-10 allograft. The NE-CS cells, which were subcutaneously inoculated into athymic mice, formed tumors with the NE phenotype. The tumors exhibited accelerated growth compared to the original NE-10 allograft. The established cell line has charact...Continue Reading

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Citations

Jan 6, 2009·International Journal of Urology : Official Journal of the Japanese Urological Association·Akira KomiyaHideki Fuse
Jan 13, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kohei HashimotoNaoya Masumori
Mar 14, 2013·Molecular Oncology·Maxime Parisotto, Daniel Metzger
Aug 9, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yongqing WangRobert J Matusik

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