PMID: 9446822Apr 4, 1998Paper

Characterization of protease-activated receptor-2 immunoreactivity in normal human tissues

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
M R D'AndreaP Andrade-Gordon

Abstract

PAR-2 is a second member of a novel family of G-protein-coupled receptors characterized by a proteolytic cleavage of the amino terminus, thus exposing a tethered peptide ligand that autoactivates the receptor. The physiological and/or pathological role(s) of PAR-2 are still unknown. This study provides tissue-specific cellular localization of PAR-2 in normal human tissues by immunohistochemical techniques. A polyclonal antibody, PAR-2C, was raised against a peptide corresponding to the amino terminal sequence SLIGKVDGTSHVTGKGV of human PAR-2. Significant PAR-2 immunoreactivity was detected in smooth muscle of vascular and nonvascular origin and stromal cells from a variety of tissues. PAR-2 was also present in endothelial and epithelial cells independent of tissue type. Strong immunolabeling was observed throughout the gastrointestinal tract, indicating a possible function for PAR-2 in this system. In the CNS, PAR-2 was localized to many astrocytes and neurons, suggesting involvement of PAR-2 in neuronal function. A role for PAR-2 in the skin was further supported by its immunolocalization in the epidermis. PAR-2C antibody exemplifies an important tool to address the physiological role(s) of PAR-2.

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·R J SantulliP Andrade-Gordon
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·S NystedtJ Sundelin
Apr 26, 1996·International Journal of Cardiology·H I MagazineK D Srivastava
Feb 14, 1997·The Journal of Biological Chemistry·M MolinoL F Brass

❮ Previous
Next ❯

Citations

Mar 27, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Eric HyunNathalie Vergnolle
Oct 6, 2011·Seminars in Immunopathology·Andrea S Rothmeier, Wolfram Ruf
May 1, 2007·Basic Research in Cardiology·Jennifer L StrandeJohn E Baker
Oct 26, 2010·Basic Research in Cardiology·Yoonjung ParkCuihua Zhang
Apr 7, 2007·Bulletin of Experimental Biology and Medicine·A M MakarovaS M Strukova
Jul 2, 2005·The Journal of Allergy and Clinical Immunology·Michael M GrunsteinSing Chuang
Aug 9, 2003·Biochemical Pharmacology·Victor E BarriosClifford D Wright
Jun 27, 2003·Microvascular Research·Barden ChanVikas P Sukhatme
Sep 24, 2002·Pharmacology & Therapeutics·Rommel S LanPeter J Henry
Feb 26, 2000·Trends in Pharmacological Sciences·T M Cocks, J D Moffatt
Dec 29, 1999·International Journal of Radiation Oncology, Biology, Physics·J WangM Hauer-Jensen
Jan 5, 2000·Bone·L A AbrahamE J Mackie
Jan 12, 2002·British Journal of Pharmacology·Carla Cicala
Oct 23, 2002·Journal of Neurochemistry·Rosario GarridoMichael Mulholland
Feb 16, 2006·The Journal of Experimental Medicine·Farshid NoorbakhshChristopher Power
Aug 7, 2003·Inflammatory Bowel Diseases·Jin-A KimYoung-Mi Lee
Apr 17, 2013·Biochemistry. Biokhimii︠a︡·T S Zamolodchikova
Oct 9, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Beihua Zhong, Donna H Wang
May 12, 2000·American Journal of Respiratory and Critical Care Medicine·F L RicciardoloP Geppetti
Nov 4, 2006·American Journal of Respiratory and Critical Care Medicine·Kazuhiko ShinagawaFrancis J Castellino
Feb 12, 2005·American Journal of Respiratory Cell and Molecular Biology·Sophie DulonMichel Chignard
Feb 21, 2003·American Journal of Respiratory Cell and Molecular Biology·Sophie DulonDominique Pidard
Jan 23, 1999·The Journal of Clinical Investigation·T D NguyenN W Bunnett

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
S NystedtJ Sundelin
Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
M R D'AndreaP Andrade-Gordon
Proceedings of the National Academy of Sciences of the United States of America
W KongNigel W Bunnett
© 2021 Meta ULC. All rights reserved