Characterization of protein factor(s) in rat bronchoalveolar lavage fluid that enhance insulin transport via transcytosis across primary rat alveolar epithelial cell monolayers.

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Rana BahhadyWei-Chiang Shen

Abstract

The aim of this study was to characterize factor(s) in rat bronchoalveolar lavage fluid (BALF) that enhance(s) insulin transport across primary rat alveolar epithelial cell monolayers (RAECM) in primary culture. BALF was concentrated 7.5-fold using the Centricon device and the retentate was used to characterize the factor(s) involved in enhancing apical-to-basolateral transport of intact 125I-insulin across various epithelial cell monolayers. These factor(s) enhanced transport of intact insulin across type II cell-like RAECM (3-fold increase) and type I cell-like RAECM (2-fold increase), but not across Caco-2 or MDCK cell monolayers. The insulin transport-enhancing factor(s) were temperature- and trypsin-sensitive. The mechanism of enhancement did not seem to involve paracellular transport or fluid-phase endocytosis, since fluxes of sodium fluorescein and FITC-dextran (70 kDa) were not affected by the factor(s) in the apical bathing fluid. BALF enhancement of intact 125I-insulin transport was abolished at 4 degrees C and in the presence of monensin, suggesting involvement of transcellular pathways. Sephacryl S-200 purification of BALF retentate, followed by LC-MS/MS, indicated that the high molecular weight (>100 kDa) fractions...Continue Reading

Citations

Feb 21, 2009·Molecular Pharmaceutics·Leena N PatelWei-Chiang Shen
Jan 11, 2003·American Journal of Respiratory and Critical Care Medicine·Heimo MairbäurlPeter Bärtsch
Feb 21, 2003·American Journal of Respiratory Cell and Molecular Biology·Sofija JovanovićAleksandar Jovanović
Sep 25, 2004·American Journal of Respiratory Cell and Molecular Biology·Kristin HeerleinHeimo Mairbäurl
Feb 8, 2005·American Journal of Respiratory Cell and Molecular Biology·Stefanie KrickFrank Rose
Jun 3, 2006·American Journal of Respiratory Cell and Molecular Biology·Diane BouvryChristine Clerici
Mar 4, 2006·American Journal of Respiratory Cell and Molecular Biology·Hans G Folkesson, Michael A Matthay
Oct 23, 2012·Journal of Cellular Physiology·Ahmed A RehmanFahim H Khan

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