Characterization of protein kinase C-mediated phosphorylation of the short cytoplasmic domain isoform of C-CAM

FEBS Letters
M EdlundB Obrink

Abstract

C-CAM is a ubiquitously expressed cell adhesion molecule belonging to the carcinoembryonic antigen family. Two co-expressed isoforms, C-CAM-L and C-CAM-S, are known, having different cytoplasmic domains both of which can be phosphorylated in vivo. Here we have characterized the PKC-mediated phosphorylation of the short cytoplasmic domain isoform, C-CAM-S. Phorbol myristyl acetate induced phosphorylation of C-CAM-S in transfected CHO cells. Using synthetic peptides and Edman degradation we identified Ser449 as the PKC-phosphorylated amino acid residue. Binding experiments with modified peptides indicated that this phosphorylation decreases the ability of the cytoplasmic domain of C-CAM-S to bind calmodulin.

References

Jan 1, 1988·Annual Review of Immunology·A F Williams, A N Barclay
Jul 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P OdinB Obrink
Feb 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K L Carraway, C A Carraway
Aug 25, 1996·Experimental Cell Research·E Ruoslahti, B Obrink
Oct 23, 1996·Biochemical and Biophysical Research Communications·O BaumD C Hixson
Oct 23, 1997·Current Opinion in Cell Biology·B Obrink
Aug 13, 2009·Molecular Cancer Therapeutics·Xiaoming XieMien-Chie Hung

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Citations

Aug 2, 2013·Cancer Metastasis Reviews·Nicole Beauchemin, Azadeh Arabzadeh
May 24, 2011·Molecular Biotechnology·Alexandra ThieleMike Schutkowski
May 21, 1999·European Journal of Biochemistry·L LuckaW Reutter
Jan 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Julia KirshnerJohn E Shively
Nov 18, 2000·Experimental Cell Research·C Wagener, S Ergün
Apr 28, 2004·Biochemical and Biophysical Research Communications·Detlef SchumannJohn E Shively
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Kai HilperRobert E W Hancock
Mar 3, 2005·Chembiochem : a European Journal of Chemical Biology·Mike SchutkowskiUlf Reimer
Apr 11, 2019·Physiology·Sonia M Najjar, Germán Perdomo
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E MatalaB Walcheck
Sep 1, 1999·European Journal of Biochemistry·Julie RobitailleNicole Beauchemin
Oct 14, 2018·International Journal of Molecular Sciences·Andrea Kristina HorstGisa Tiegs

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