Characterization of protein release through glucose-sensitive hydrogel membranes

Biomaterials
A A Obaidat, K Park

Abstract

Glucose-sensitive phase-reversible hydrogels have been prepared based on the specific interaction between polymer-bound glucose and concanavalin A (Con-A). The main goal of this study was to characterize the release of model proteins (insulin and lysozyme) through the hydrogel membrane as the free glucose concentration in the environment was changed. The diffusion of the model proteins through the hydrogel membrane was examined using a diffusion cell. Porous poly(hydroxyethyl methacrylate) (PHEMA) membranes were used to sandwich the mixture of glucose-containing polymers and Con-A in between the donor and receptor chambers. The porous PHEMA membranes allowed diffusion of glucose, insulin and lysozyme, while preventing loss of glucose-containing polymers and Con-A in the sol state. The release rate of model proteins through the glucose-sensitive hydrogel membrane was dependent on the concentration of free glucose. The release rate of the proteins did not remain constant, however, due to the change in free glucose concentration resulting from diffusion of glucose from the receptor chamber to the donor chamber. This study demonstrated the possibility that the glucose-sensitive phase-reversible hydrogels can be used to regulate the...Continue Reading

References

Aug 15, 1972·Biochemistry·B A CunninghamG M Edelman
Jan 1, 1970·International Archives of Allergy and Applied Immunology·W H Beckert, M M Sharkey
Oct 1, 1970·Experimental Cell Research·A E Powell, M A Leon
Mar 1, 1983·Journal of Pharmaceutical Sciences·S SatoS W Kim
Sep 1, 1983·Journal of Biomedical Materials Research·G F KlompW H Dobelle
Sep 1, 1983·Journal of Biomedical Materials Research·S H RonelW H Dobelle
Jul 1, 1980·Diabetologia·W D LougheedA M Albisser
Jan 1, 1994·Journal of Biomaterials Science. Polymer Edition·K NakamaeA S Hoffman
Sep 1, 1996·Journal of Molecular Recognition : JMR·S J Lee, K Park
Apr 25, 1993·Biotechnology and Bioengineering·J E MorrisR R Fisher

❮ Previous
Next ❯

Citations

Apr 23, 2009·Pharmaceutical Research·S LitvinchukW Meier
Jul 17, 1999·Trends in Biotechnology·I Y Galaev, B Mattiasson
Jun 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·A TangJ Kopecek
Jul 14, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·Y ParkV C Yang
Nov 2, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·J J Kim, K Park
Dec 18, 2001·Advanced Drug Delivery Reviews·Y Qiu, K Park
Jan 5, 2002·Advanced Drug Delivery Reviews·Takashi MiyataKatsuhiko Nakamae
Jan 5, 2002·Advanced Drug Delivery Reviews·Akihiko Kikuchi, Teruo Okano
Jan 5, 2002·Advanced Drug Delivery Reviews·Mark E ByrneNicholas A Peppas
Oct 24, 2002·Advanced Drug Delivery Reviews·S Sershen, J West
Sep 19, 2003·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jindrich Kopecek
Mar 21, 2001·Biotechnology & Genetic Engineering Reviews·G AdamsM J Taylor
Mar 23, 2001·Journal of Biomaterials Science. Polymer Edition·R A GemeinhartK Park
Aug 17, 2002·Journal of Biomaterials Science. Polymer Edition·Hasoo SeongKinam Park
Feb 9, 2005·Journal of Biomaterials Science. Polymer Edition·Jun Bae LeeTae Gwan Park
Nov 28, 2002·Clinical Pharmacokinetics·Yie W Chien, Senshang Lin
Jan 1, 2010·Sensors·Kamila GawelBjørn Torger Stokke
Mar 20, 2010·AAPS PharmSciTech·Rubina P ShaikhShivaan Cooppan
Mar 15, 2005·Nature Materials·Jason D EhrickSylvia Daunert
Oct 11, 2015·Journal of Drug Targeting·Matthew J Webber, Daniel G Anderson
Jul 2, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ronald A Siegel
Aug 16, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Changhong ZhangThomas Boland
Aug 27, 2004·Biotechnology and Bioengineering·M TangJ Hubble
Nov 18, 2009·Journal of Polymer Science. Part A, Polymer Chemistry·Jindřich Kopeček
Feb 6, 2003·Biotechnology and Bioengineering·M TangJ Hubble
Jul 11, 2013·Macromolecular Bioscience·Weitai Wu, Shuiqin Zhou
Jun 1, 2012·Macromolecular Rapid Communications·Raphael J GübeliWilfried Weber
Dec 22, 2006·Journal of Colloid and Interface Science·Anna SalvatiDaniel Topgaard
Mar 16, 2011·Biotechnology and Bioengineering·Emma PiacentiniLidietta Giorno
Jul 2, 2005·Expert Opinion on Emerging Drugs·C Alexander
Sep 8, 2004·Advanced Drug Delivery Reviews·J Zachary Hilt, Mark E Byrne
Dec 1, 1999·The Journal of Pharmacy and Pharmacology·S TannaG Adams
Jun 29, 2004·Trends in Pharmacological Sciences·Gorka OriveJosé Luis Pedraz
Dec 9, 2010·Drug Delivery·B R GandhiP P Gandhi
Jan 13, 2017·Journal of Biomaterials Science. Polymer Edition·Jinku XuYongchun Zhang
Jan 4, 2019·Advanced Healthcare Materials·Michael A VandenBerg, Matthew J Webber
Jul 10, 2019·Biotechnology Journal·Cláudia S M FernandesAna C A Roque
Aug 20, 2019·Advanced Materials·Jinqiang WangZhen Gu
Feb 27, 2013·Journal of Diabetes Science and Technology·Achim Josef MüllerGerd Uwe Auffarth
Dec 1, 2016·Nature Reviews. Materials·Jianyu Li, David J Mooney

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.