Characterization of protein-tyrosine phosphatases that dephosphorylate the high affinity IgE receptor.

The Journal of Biological Chemistry
S Y Mao, H Metzger

Abstract

An early event that follows aggregation of the high affinity receptor for IgE (FcepsilonRI) is the phosphorylation of protein tyrosines, especially those on the beta- and gamma-subunits of the receptor. Disaggregation of the receptors leads to their rapid dephosphorylation, but even stably aggregated receptors undergo continual rounds of phosphorylation and dephosphorylation. We developed assays to study dephosphorylation of the receptors and other cellular proteins. Whole cell extracts dephosphorylated both subunits of the receptors rapidly and were as active against aggregated as against disaggregated FcepsilonRI. Upon disaggregation, the in vivo dephosphorylation of the FcepsilonRI and several other proteins followed first-order kinetics with closely similar rate constants despite substantial differences in the extent of phosphorylation. These results suggest that the level of phosphorylation of FcepsilonRI is largely controlled by the aggregation-induced action of kinase(s) and not from changes in susceptibility to or activity of the phosphatases. Much of the total phosphatase is lost when the cells are permeabilized, but the rate of dephosphorylation of disaggregated FcepsilonRI was comparable in intact and permeabilized c...Continue Reading

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Citations

Sep 10, 2002·Molecular Immunology·Henry MetzgerChikako Torigoe
Sep 10, 2002·Molecular Immunology·Byron GoldsteinCarla Wofsy
Jul 9, 1999·Immunology Letters·H MetzgerC Wofsy
Jun 3, 2004·Nature Reviews. Immunology·Byron GoldsteinWilliam S Hlavacek
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Aug 23, 2002·Immunological Reviews·Henry Metzger
Nov 17, 2009·Immunological Reviews·Damiana Alvarez-ErricoJuan Rivera
Aug 2, 2005·Biochimica Et Biophysica Acta·David HolowkaBarbara Baird
Jul 4, 2017·PloS One·Donald MacGlashan
Nov 13, 2004·The Journal of Biological Chemistry·Ryan M YoungBarbara Baird
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Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chikako TorigoeByron Goldstein
Aug 25, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ambarish NagByron Goldstein

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