PMID: 9420293Jan 7, 1998Paper

Characterization of provirus clones of simian foamy virus type 1

Journal of Virology
A Mergia, M Wu

Abstract

We have cloned proviral DNA of simian foamy virus type 1 (SFV-1) from linear unintegrated DNA (pSFV-1). Transfection of pSFV-1 induces cytopathology in several cell lines with supernatants from the transfected cell culture containing infectious viral particles. Electron microscopy of the transfected cells revealed foamy virus particles. Deletion analysis of pSFV-1 indicated that the transcriptional transactivator (tas) gene located between env and the long terminal repeat is critical for virus replication, whereas the second open reading frame (ORF-2) in this region is dispensable. Although the tas and ORF-2 regions of foamy viruses have significantly diverged, the results presented here suggested that the gene products have similar functions. Recombinant pSFV-1 containing the cat gene was able to transduce the heterologous gene, indicating the utility of SFV-1 as a vector. An infectious clone of SFV-1 which is distantly related to the human foamy virus will provide a means to understand the biology of this unique group of viruses.

References

Feb 1, 1992·Virology·R RenneD Neumann-Haefelin
May 30, 1991·Gene·J J KupiecF Galibert
Oct 1, 1991·Virology·A Mergia, P A Luciw
Feb 25, 1990·Nucleic Acids Research·A RethwilmV ter Meulen
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A RethwilmV ter Meulen
Oct 1, 1994·The Journal of General Virology·H HahnA Rethwilm
Jun 1, 1993·Journal of Virology·M L GironR Emanoil-Ravier
Sep 1, 1993·Journal of Virology·G BaunachA Rethwilm
Jan 1, 1996·Journal of Virology·D W Russell, A D Miller

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Citations

Oct 15, 2003·Journal of Virology·Jacqueline RoyAxel Rethwilm
Nov 8, 2013·Journal of Virology·Carolyn R StenbakMaxine L Linial
May 1, 2013·Emerging Microbes & Infections·Mostafa M FeerozLisa Jones-Engel
Mar 28, 2008·Journal of Virology·Melvyn W YapJonathan P Stoye
Mar 29, 2003·Journal of Virology·Marcus Picard-MaureauDirk Lindemann
Apr 3, 1998·Journal of Virology·M WuA Mergia
Apr 10, 1999·Journal of Virology·M Wu, A Mergia
Sep 2, 2011·Cold Spring Harbor Protocols·Jeonghae Park, Ayalew Mergia

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