PMID: 9660140Jul 11, 1998Paper

Characterization of purified metallo- and cysteine proteases from the pathogenic haemoflagellate Cryptobia salmositica Katz 1951

Parasitology Research
X Zuo, P T Woo

Abstract

The purified metalloprotease and the partially purified cysteine protease from pathogenic Cryptobia salmositica were characterized. Using haemoglobin gel electrophoresis, we detected five enzymatic bands in crude parasite lysate; one protease (200 kDa) yielded a metalloprotease band and other four, cysteine protease bands (97, 70, 66 and 49 kDa). Both the metalloprotease and the cysteine protease had high levels of proteolytic activity against azocasein, haemoglobin and fibrinogen. The metalloprotease had high levels of activity against azocoll and gelatin but a low degree of activity against albumin. In contrast, the cysteine protease had extensive activity against albumin but low levels of activity against azocoll and gelatin. The metallo- and cysteine proteases had no activity against Pz-peptide, a specific substrate for bacterial collagenase. The optimal pH for the metalloprotease and the cysteine protease was 7.0 and 5.0, respectively. The metalloprotease was inhibited by metal-chelating agents and excess of zinc ions but was activated by calcium ions. The cysteine protease was inhibited by thiol-blocking agents. The natural antiprotease alpha2-macroglobulin, but not alpha1-protease inhibitor, inhibited the activity of bot...Continue Reading

Citations

Dec 16, 2006·Parasitology Research·Palmy R JesudhasanPatrick T K Woo
Nov 18, 2006·Parasitology Research·Palmy R R JesudhasanPatrick T K Woo
Jun 7, 2005·Developmental and Comparative Immunology·Geert F WiegertjesJörn P Scharsack
May 4, 2001·International Journal for Parasitology·P T Woo
Oct 12, 2013·Diseases of Aquatic Organisms·Itziar EstensoroAriadna Sitjà-Bobadilla
Jul 23, 2013·Developmental and Comparative Immunology·Patrick T K Woo, Bernadette F Ardelli
Jan 7, 2010·Journal of Biomedicine & Biotechnology·Patrick T K Woo
Feb 22, 2005·The New Phytologist·Caroline Pak, Wouter G van Doorn
Sep 10, 2009·The Journal of Eukaryotic Microbiology·Claudia M D'Avila-LevyMarta H Branquinha
Jan 9, 2004·Journal of Fish Diseases·P T K Woo
Jul 31, 2007·Developmental and Comparative Immunology·Dalia F OnaraGeert F Wiegertjes
Jun 16, 2012·International Journal of Aging & Human Development·Xue BaiNelson Chow

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