Characterization of Purple Acid Phosphatase Family and Functional Analysis of GmPAP7a /7b Involved in Extracellular ATP Utilization in Soybean

Frontiers in Plant Science
Shengnan ZhuJiang Tian

Abstract

Low phosphate (Pi) availability limits crop growth and yield in acid soils. Although root-associated acid phosphatases (APases) play an important role in extracellular organic phosphorus (P) utilization, they remain poorly studied in soybean (Glycine max), an important legume crop. In this study, dynamic changes in intracellular (leaf and root) and root-associated APase activities were investigated under both Pi-sufficient and Pi-deficient conditions. Moreover, genome-wide identification of members of the purple acid phosphatase (PAP) family and their expression patterns in response to Pi starvation were analyzed in soybean. The functions of both GmPAP7a and GmPAP7b, whose expression is up regulated by Pi starvation, were subsequently characterized. Phosphate starvation resulted in significant increases in intracellular APase activities in the leaves after 4 days, and in root intracellular and associated APase activities after 1 day, but constant increases were observed only for root intracellular and associated APase activities during day 5-16 of P deficiency in soybean. Moreover, a total of 38 GmPAP members were identified in the soybean genome. The transcripts of 19 GmPAP members in the leaves and 17 in the roots were upregu...Continue Reading

References

Jan 18, 2003·Biochimica Et Biophysica Acta·Rumi KaidaTakako S Kaneko
Sep 18, 2004·Plant Biology·P ZimmermannM Bucher
May 9, 2006·Plant Physiology·Rajendra BariWolf-Rüdiger Scheible
Mar 21, 2007·Archives of Biochemistry and Biophysics·Mariusz Olczak, Teresa Olczak
Feb 21, 2009·Science·Daniel J ConleyGene E Likens
Oct 13, 2009·Plant Signaling & Behavior·Claudia Anahí Pérez TorresLuis Herrera Estrella
Jan 12, 2011·Plant Physiology·Giuseppe DionisioHenrik Brinch-Pedersen
Mar 5, 2011·Annual Review of Plant Biology·Tzyy-Jen Chiou, Shu-I Lin
Mar 27, 2012·Current Opinion in Biotechnology·Jiang TianHong Liao
Jun 14, 2012·The New Phytologist·Erik J VeneklaasJohn A Raven
Jan 15, 2014·Journal of Integrative Plant Biology·Zhaoliang ZhangWilliam J Lucas
Jul 19, 2014·Current Opinion in Plant Biology·Cuiyue LiangHong Liao
Jun 5, 2015·Frontiers in Plant Science·Eliécer González-MuñozRuairidh J H Sawers
Apr 1, 2009·Plant Science : an International Journal of Experimental Plant Biology·Xue-Feng MaZeng-Yu Wang
May 22, 2017·Current Opinion in Plant Biology·Steffen Abel
Jul 22, 2017·Current Opinion in Biotechnology·Byung-Kook HamWilliam J Lucas
Sep 11, 2017·Current Opinion in Biotechnology·Ji-Yul JungYves Poirier
Oct 11, 2017·Journal of Experimental Botany·Ying-Bin XueGifford Miriam
Apr 14, 2018·Plant Science : an International Journal of Experimental Plant Biology·Liangsheng Wang, Dong Liu

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Methods Mentioned

BETA
protein modification
transgenic
confocal microscopy
glycosylation

Software Mentioned

Zen2011
ExPASy
ClustalX
TBtools
MEGA
Excel
SMART tool
NetNGlyc
SPSS
TargetP

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