Characterization of putative drug resistant biomarkers in Plasmodium falciparum isolated from Ghanaian blood donors

BMC Infectious Diseases
Enoch AninagyeiDesmond Omane Acheampong

Abstract

Plasmodium falciparum parasites, which could harbour anti-malaria drug resistance genes, are commonly detected in blood donors in malaria-endemic areas. Notwithstanding, anti-malaria drug resistant biomarkers have not been characterized in blood donors with asymptomatic P. falciparum infection. A total of 771 blood donors were selected from five districts in the Greater Accra Region, Ghana. Each donor sample was screened with malaria rapid diagnostic test (RDT) kit and parasitaemia quantified microscopically. Dried blood spots from malaria positive samples were genotyped for P. falciparum chloroquine resistance transporter (Pfcrt), P. falciparum multi-drug resistance (Pfmdr1), P. falciparum dihydropteroate-synthetase (Pfdhps), P. falciparum dihydrofolate-reductase (Pfdhfr) and Kelch 13 propeller domain on chromosome 13 (Kelch 13) genes. Of the 771 blood donors, 91 (11.8%) were positive by RDT. Analysis of sequence reads indicated successful genotyping of Pfcrt, Pfmdr1, Pfdhfr, Pfdhps and Kelch 13 genes in 84.6, 81.3, 86.8, 86.9 and 92.3% of the isolates respectively. Overall, 21 different mutant haplotypes were identified in 69 isolates (75.8%). In Pfcrt, CVIET haplotype was observed in 11.6% samples while in Pfmdr1, triple mut...Continue Reading

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Citations

Jun 20, 2021·International Journal for Parasitology. Drugs and Drug Resistance·Leonard NdwigaLynette Isabella Ochola-Oyier
Jul 27, 2021·Medicinal Research Reviews·Hannah Michaela BehrensTobias Spielmann
Jul 28, 2021·Expert Opinion on Pharmacotherapy·Erik KoehneAndrea Kreidenweiss

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Methods Mentioned

BETA
genotyping
blood collection

Software Mentioned

excel
SPSS
fastq
Bioedit

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