PMID: 6166393May 1, 1981Paper

Characterization of rat colonic epithelial cell populations

Cell Differentiation
R PatnaikP P Nair

Abstract

Colonic epithelial cells from male Sprague-Dawley rats were isolated by incubating everted colon with hyaluronidase suspended in Puck's saline F with an average cell yield of 120 x 10(6). These cells were fractionated by discontinuous Ficoll gradient and by short-term cell culture techniques. Centrifugation of isolated cells on discontinuous Ficoll gradient (15-35%) yielded populations differing in their proliferative activity. Additionally, a short-term cell culture technique was standardized to fractionate these cells according to their proliferating activity as judged by their DNA synthesis and thymidine kinase activity. Viability of these cells were judged by trypan blue exclusion, capacity to oxidize glucose and incorporation of precursors into protein DNA, RNA and glycoproteins. These fractionated cells were examined and identified by cytological studies. Cells showing proliferative activity sedimented at heavier regions of the Ficoll gradient, and the majority of these cells attached to the surface under conditions of short-term culture. Columnar mature absorptive cells and mucus-secreting goblet cells that showed very little proliferative activity sedimented at lighter zones of the Ficoll gradient and a major portion of...Continue Reading

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Citations

Aug 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Mathie LorrotMonique Vasseur
Jan 1, 1984·Pathology·R K Kumar, A W Lykke
Feb 1, 1987·Applied and Environmental Microbiology·D D Whitt, D C Savage

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