PMID: 18719742Aug 23, 2008Paper

Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
A MotaM J Martins

Abstract

Alkaline phosphatase (ALP) is important in calcification and its expression seems to be associated with the inflammatory process. We investigated the in vitro acute effects of compounds used for the prevention or treatment of cardiovascular diseases on total ALP activity from male Wistar rat heart homogenate. ALP activity was determined by quantifying, at 410 nm, the p-nitrophenol released from p-nitrophenylphosphate (substrate in Tris buffer, pH 10.4). Using specific inhibitors of ALP activity and the reverse transcription-polymerase chain reaction, we showed that the rat heart had high ALP activity (31.73 +/- 3.43 nmol p-nitrophenol.mg protein-1.min-1): mainly tissue-nonspecific ALP but also tissue-specific intestinal ALP type II. Both ALP isoenzymes presented myocardial localization (striated pattern) by immunofluorescence. ALP was inhibited a) strongly by 0.5 mM levamisole, 2 mM theophylline and 2 mM aspirin (91, 77 and 84%, respectively) and b) less strongly by 2 mM L-phenylalanine, 100 mL polyphenol-rich beverages and 0.5 mM progesterone (24, 21 to 29 and 11%, respectively). beta-estradiol and caffeine (0.5 and 2 mM) had no effect; 0.5 mM simvastatin and 2 mM atenolol activated ALP (32 and 36%, respectively). Propranolol ...Continue Reading

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Citations

Dec 3, 2008·Pediatric Cardiology·Maria João Martins, Isabel Azevedo
Apr 5, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T G G ZotzA D L Moser
Jun 5, 2014·Molecular and Cellular Biochemistry·Xiaohong Tracey GanMorris Karmazyn
Aug 18, 2012·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Birgit LohbergerNorbert Jakse
Mar 24, 2009·International Journal of Cardiology·Maria João Martins, Isabel Azevedo
Jul 13, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Yuan WangBradley M Palmer

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