Characterization of Rat ILCs Reveals ILC2 as the Dominant Intestinal Subset.

Frontiers in Immunology
Ahmed AbidiJérôme Martin

Abstract

Innate lymphoid cells (ILCs) are tissue-resident lymphocytes that lack antigen-specific receptors and exhibit innate effector functions such as cytokine production that play an important role in immediate responses to pathogens especially at mucosal sites. Mouse and human ILC subsets have been extensively characterized in various tissues and in blood. In this study, we present the first characterization of ILCs and ILC subsets in rat gut and secondary lymphoid organs using flow cytometry and single cell RNA sequencing. Our results show that phenotype and function of rat ILC subsets are conserved as compared to human and mouse ILCs. However, and in contrast to human and mouse, our study unexpectedly revealed that ILC2 and not ILC3 was the dominant ILC subset in the rat intestinal lamina propria. ILC2 predominance in the gut was independent of rat strain, sex or housing facility. In contrast, ILC3 was the predominant ILC subset in mesenteric lymph nodes and Peyer patches. In conclusion, our study demonstrates that in spite of highly conserved phenotype and function between mice, rat and humans, the distribution of ILC subsets in the intestinal mucosa is dependent on the species likely in response to both genetic and environmental...Continue Reading

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Citations

Mar 21, 2021·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Kazuki HoriuchiRyota Hokari

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Datasets Mentioned

BETA
GSE143920

Methods Mentioned

BETA
flow
scRNAseq
FACS
RNAseq
scRNA-seq
flow cytometry
PMA

Software Mentioned

scDissector
R
Treestar
GraphPad Prism
CellRanger
FACSDiva
BD
DESeq2
Seurat R package
Flow Jo

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