Characterization of rat iodothyronine sulfotransferases

American Journal of Physiology. Endocrinology and Metabolism
M H KesterT J Visser

Abstract

Sulfation appears to be an important pathway for the reversible inactivation of thyroid hormone during fetal development. The rat is an often used animal model to study the regulation of fetal thyroid hormone status. The present study was done to determine which sulfotransferases (SULTs) are important for iodothyronine sulfation in the rat, using radioactive T4, T3, rT3, and 3,3'-T2 as substrates, 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as cofactor, and rat liver, kidney and brain cytosol, and recombinant rat SULT1A1, -1B1, -1C1, -1E1, -2A1, -2A2, and -2A3 as enzymes. Recombinant rat SULT1A1, -1E1, -2A1, -2A2, and -2A3 failed to catalyze iodothyronine sulfation. For all tissue SULTs and for rSULT1B1 and rSULT1C1, 3,3'-T2 was by far the preferred substrate. Apparent Km values for 3,3'-T2 amounted to 1.9 microM in male liver, 4.4 microM in female liver, 0.76 microM in male kidney, 0.23 microM in male brain, 7.7 microM for SULT1B1, and 0.62 microM for SULT1C1, whereas apparent Km values for PAPS showed less variation (2.0-6.9 microM). Sulfation of 3,3'-T2 was inhibited dose dependently by other iodothyronines, with similar structure-activity relationships for most enzymes except for the SULT activity in rat brain. The apparen...Continue Reading

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Citations

Nov 4, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·David T SzaboLinda S Birnbaum
Nov 17, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Katie B PaulKevin M Crofton
Mar 27, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Béatrice B RoquesCatherine Viguié
Nov 24, 2004·Drug Metabolism Reviews·Frederick C Kauffman
Sep 14, 2013·Drug Metabolism Reviews·Melissa Runge-Morris, Thomas A Kocarek
Nov 2, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Vicki M RichardsonMichael J Devito
Dec 29, 2009·General and Comparative Endocrinology·Farhana Babli Rahman, Kiyoshi Yamauchi
Jan 26, 2008·International Journal of Andrology·Kevin M Crofton
Jan 10, 2006·Methods in Enzymology·Nandita ShangariPeter J O'Brien
Jan 23, 2017·Molecular and Cellular Endocrinology·Anne H van der SpekAnita Boelen
Sep 1, 2005·Thyroid : Official Journal of the American Thyroid Association·Sing-Yung WuInder J Chopra
Feb 8, 2008·Bioscience, Biotechnology, and Biochemistry·Kenji HattoriHiro-omi Tamura
May 5, 2012·The Journal of Biological Chemistry·Edward L MertzAntonella Forlino
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Dec 6, 2005·Pediatric Research·Sing-Yung WuDelbert A Fisher
Jul 6, 2004·Archives of Biochemistry and Biophysics·Lesley A WilsonMichael W H Coughtrie

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