Abstract
Sulfation appears to be an important pathway for the reversible inactivation of thyroid hormone during fetal development. The rat is an often used animal model to study the regulation of fetal thyroid hormone status. The present study was done to determine which sulfotransferases (SULTs) are important for iodothyronine sulfation in the rat, using radioactive T4, T3, rT3, and 3,3'-T2 as substrates, 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as cofactor, and rat liver, kidney and brain cytosol, and recombinant rat SULT1A1, -1B1, -1C1, -1E1, -2A1, -2A2, and -2A3 as enzymes. Recombinant rat SULT1A1, -1E1, -2A1, -2A2, and -2A3 failed to catalyze iodothyronine sulfation. For all tissue SULTs and for rSULT1B1 and rSULT1C1, 3,3'-T2 was by far the preferred substrate. Apparent Km values for 3,3'-T2 amounted to 1.9 microM in male liver, 4.4 microM in female liver, 0.76 microM in male kidney, 0.23 microM in male brain, 7.7 microM for SULT1B1, and 0.62 microM for SULT1C1, whereas apparent Km values for PAPS showed less variation (2.0-6.9 microM). Sulfation of 3,3'-T2 was inhibited dose dependently by other iodothyronines, with similar structure-activity relationships for most enzymes except for the SULT activity in rat brain. The apparen...Continue Reading
References
Jul 1, 1992·Journal of Biochemistry·D W GongR Kato
May 1, 1992·The Journal of Clinical Endocrinology and Metabolism·S W SpauldingJ A Roth
Jun 1, 1992·Pediatric Research·F SantiniG N Chua Teco
Apr 1, 1991·Endocrinology·V A GaltonD L St Germain
Apr 1, 1988·Endocrinology·M P KungJ A Roth
May 1, 1988·Endocrinology·W F YoungR M Weinshilboum
Aug 11, 1995·The Journal of Biological Chemistry·C C KiehlbauchD P Ringer
Feb 1, 1995·Thyroid : Official Journal of the American Thyroid Association·R J AndersonD K Liebentritt
Jan 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·J L FalanyC N Falany
Jun 1, 1994·Chemico-biological Interactions·A CzichH R Glatt
Jun 1, 1994·Chemico-biological Interactions·K IwasakiH Noguchi
May 16, 1994·FEBS Letters·M MorenoT J Visser
Jun 1, 1993·The Journal of Clinical Endocrinology and Metabolism·F SantiniI J Chopra
Dec 22, 1995·The Journal of Biological Chemistry·Y SakakibaraM C Liu
Jul 17, 1996·Biochimica Et Biophysica Acta·B A Rikke, A K Roy
Jun 1, 1996·The Journal of Clinical Endocrinology and Metabolism·J M Koopdonk-KoolT J Visser
Feb 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M A Runge-Morris
Mar 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C N Falany
May 1, 1997·The International Journal of Biochemistry & Cell Biology·Y ArakiM C Liu
Dec 6, 1997·Endocrinology·E KapteinT J Visser
Jan 10, 1998·The Journal of Physiology·M MorenoF Goglia
May 5, 1998·Chemico-biological Interactions·H GlattY Yamazoe
May 5, 1998·Chemico-biological Interactions·T J VisserM W Coughtrie
Feb 2, 1999·Endocrinology·J M BatesV A Galton
Apr 9, 1999·The Journal of Clinical Investigation·V A GaltonD L St Germain
Apr 13, 1999·The Journal of Clinical Endocrinology and Metabolism·M H KesterT J Visser
Jun 11, 1999·Japanese Journal of Pharmacology·K FujitaY Yamazoe
Jun 22, 1999·Biological & Pharmaceutical Bulletin·K FujitaY Yamazoe
Jul 15, 1999·The Journal of Clinical Endocrinology and Metabolism·M H KesterT J Visser
Nov 15, 2000·Biochemical Pharmacology·X LiR J Anderson
Aug 24, 2001·Pediatric Research·S Y WuD H Polk
Dec 12, 2001·The Journal of Endocrinology·X LiR J Anderson
Nov 20, 2002·The Pharmacogenomics Journal·M W H Coughtrie
Feb 1, 1996·Endocrine·T J VisserW J de Greef
Citations
Oct 29, 2014·The Journal of Endocrinology·E M de VriesA Boelen
Nov 4, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·David T SzaboLinda S Birnbaum
Nov 17, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Katie B PaulKevin M Crofton
Mar 27, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Béatrice B RoquesCatherine Viguié
Nov 24, 2004·Drug Metabolism Reviews·Frederick C Kauffman
Sep 14, 2013·Drug Metabolism Reviews·Melissa Runge-Morris, Thomas A Kocarek
Nov 2, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Vicki M RichardsonMichael J Devito
Jun 5, 2012·Toxicology·Katie B PaulKevin M Crofton
Dec 3, 2015·Molecular and Cellular Endocrinology·L PréauB A Demeneix
Dec 29, 2009·General and Comparative Endocrinology·Farhana Babli Rahman, Kiyoshi Yamauchi
Jan 26, 2008·International Journal of Andrology·Kevin M Crofton
Aug 22, 2013·Biochemical Pharmacology·Béatrice B RoquesPascal G P Martin
Jan 10, 2006·Methods in Enzymology·Nandita ShangariPeter J O'Brien
Jul 28, 2005·The FEBS Journal·Shin YasudaMing-Cheh Liu
Jan 23, 2017·Molecular and Cellular Endocrinology·Anne H van der SpekAnita Boelen
Jun 30, 2010·Toxicologic Pathology·Daniela EnnulatLaura Boone
Sep 1, 2005·Thyroid : Official Journal of the American Thyroid Association·Sing-Yung WuInder J Chopra
Feb 8, 2008·Bioscience, Biotechnology, and Biochemistry·Kenji HattoriHiro-omi Tamura
May 5, 2012·The Journal of Biological Chemistry·Edward L MertzAntonella Forlino
Feb 17, 2005·Endocrinology·Ann Marie Zavacki, P Reed Larsen
Sep 23, 2014·Endocrinology·E M de VriesA Boelen
Dec 10, 2009·Endocrinology·Marija Trajkovic-ArsicHeike Heuer
Nov 27, 2004·Endocrinology·Mohammed QatananiDavid D Moore
Dec 6, 2005·Pediatric Research·Sing-Yung WuDelbert A Fisher
Jun 3, 2021·Critical Reviews in Toxicology·Sue MartyBennard van Ravenzwaay
Jul 6, 2004·Archives of Biochemistry and Biophysics·Lesley A WilsonMichael W H Coughtrie