PMID: 6989596Feb 1, 1980Paper

Characterization of rat-liver microsomal glutathione S-transferase activity

European Journal of Biochemistry
R MorgensternL Ernster

Abstract

Rat liver microsomes were shown to catalyze the conjugation of 1-chloro-2,4-dinitrobenzene with glutathione and this activity has been characterized. It cannot be removed from the microsomes by washing or other procedures which release loosely bound material from membranes. The microsomal glutathione S-transferase can be activated up to eight fold by treatment with N-ethylmaleimide. This activation also affects the apparent Km of the enzyme(s) for both glutathione and 1-chloro-2,4-dinitrobenzene. Upon subcellular fractionation of the liver the N-ethylmaleimide-activateable glutathione S-transferase distributes in the same manner as a marker for the endoplasmic reticulum and unlike markers for the other organelles and for the cytoplasm. Treatment of microsomes with proteases revealed that the enzyme is at least partially exposed on the cytoplasmic surface of the endoplasmic reticulum. Finally, three inducers of drug-metabolizing systems-i.e. phenobarbital, methylcholanthrene, and trans-stilbene oxide-all increase the activity of the cytoplasmic glutathione S-transferases, but they do not affect the microsomal activity. These and other considerations indicate that the microsomal glutathione S-transferase(s) is distinct from the c...Continue Reading

References

Dec 29, 1975·Biochimica Et Biophysica Acta·J W Depierre, G Dallner
Jan 1, 1977·Current Topics in Cellular Regulation·C J Masters
Apr 13, 1979·Biochemical and Biophysical Research Communications·R MorgensternL Ernster
Aug 1, 1973·Analytical Biochemistry·O TangenS Orrenius
Jun 1, 1961·The Biochemical Journal·J BoothP Sims

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Citations

Dec 2, 1992·Molecular and Cellular Biochemistry·J Datta, T B Samanta
Jan 1, 1991·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·N A Shi, Y G Liu
Aug 21, 2007·Parasitology Research·Gabriela NavaAgustín Plancarte
Jan 1, 1987·Chemico-biological Interactions·I M RietjensP J van Bladeren
Jan 1, 1991·Pharmacology & Therapeutics·P J van Bladeren, B van Ommen
May 1, 1987·Cancer Letters·H WallinA M Jeffre
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K LarsenB Mannervik
Jan 1, 1996·Free Radical Biology & Medicine·F AntunesR E Pinto
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T YoshimotoK F Austen
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·R ZettlK Palme
Oct 6, 1997·Journal of Toxicology and Environmental Health·G H Gunasena, M F Kanz
Jan 1, 1989·Drug Metabolism and Drug Interactions·T Igarashi, T Satoh
Mar 5, 2008·The Journal of Parasitology·Rumana Ahmad, Arvind K Srivastava
Dec 8, 1998·The International Journal of Angiology : Official Publication of the International College of Angiology, Inc·G A LosaJ C Farine
Apr 19, 2011·Drug Metabolism Reviews·Ralf MorgensternKatarina Johansson
Mar 1, 1988·Archives internationales de physiologie et de biochimie·P J Dierickx
Apr 8, 2009·Analytical Biochemistry·Johan AlanderRalf Morgenstern
Sep 11, 2004·Chemico-biological Interactions·Jie ZhangYijia Lou
Jul 6, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Maxwell Afari GyamfiYoko Aniya
Aug 10, 1994·Forensic Science International : Synergy·M Yamazaki, C Wakasugi
Apr 21, 2005·Chemico-biological Interactions·Jie ZhangYijia Lou
Oct 24, 1991·Biochemical Pharmacology·L J ShoreG B Odell
Nov 9, 2004·Structure·Ingeborg Schmidt-KreyWerner Kühlbrandt
Jan 1, 1988·CRC Critical Reviews in Biochemistry·B Mannervik, U H Danielson
Aug 14, 1981·Biochemical and Biophysical Research Communications·C C ReddyE J Massaro
Nov 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Joshua J EmrickDavid Julius
Oct 15, 1993·European Journal of Biochemistry·F PiemonteG Federici

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