Characterization of rat testes mitochondrial retinoylating system and its partial purification

Journal of Bioenergetics and Biomembranes
E Cione, G Genchi

Abstract

Retinoylation (retinoic acid acylation), a posttranslational modification of proteins occurring in a variety of eukariotic cell lines both in vivo and in vitro, was studied in rat testes mitochondria. all-trans-Retinoic acid, a highly active form of vitamin A in inducing cellular differentiation, is incorporated covalently into proteins of rat testes mitochondria. The maximum retinoylation activity of rat testes mitochondrial proteins was 21.6 pmoles mg protein(-1) 90 min(-1) at 37 degrees C. The activation energy was 44 kJ mol(-1) from 5 to 37 degrees C. The retinoylation activity had a pH optimum of 7.5. The retinoylation process was specific for the presence of ATP, ADP, and GTP (even if only 30% of the control). The half saturation constant (Km) was 0.69 microM for all-trans-retinoic acid, while the inhibition constant (Ki) was 1.5 microM for 13-cis-retinoic acid. Retinoylation was not inhibited by high concentrations of myristic acid (MA) and palmitic acid (PA), indicating that retinoylation and acylation reactions involved different rat testes mitochondrial proteins. The ATP or CoASH saturation curves of retinoylation reaction showed sigmoidal behavior with apparent half saturation constants (K0.5) of 6.5 mM ATP and 40.6 ...Continue Reading

Citations

May 21, 2005·Journal of Bioenergetics and Biomembranes·Erika CioneGiuseppe Genchi
Jun 13, 2009·Journal of Bioenergetics and Biomembranes·Attilio PingitoreGiuseppe Genchi
May 22, 2010·Journal of Bioenergetics and Biomembranes·Valentina SenatoreGiuseppe Genchi
Apr 17, 2007·Journal of Bioenergetics and Biomembranes·Erika CioneGiuseppe Genchi
Nov 4, 2008·Biochimica Et Biophysica Acta·E CioneG Genchi
Dec 30, 2014·Molecular Aspects of Medicine·Alessandra di MasiClara Nervi
Oct 6, 2009·The International Journal of Biochemistry & Cell Biology·Erika CioneGiuseppe Genchi
Nov 8, 2005·Journal of Biochemistry·Yoshinori KuboNoriko Takahashi

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