Characterization of recombinant and brain neuropsin, a plasticity-related serine protease.

The Journal of Biological Chemistry
C ShimizuS Shiosaka

Abstract

Activity-dependent changes in neuropsin gene expression in the hippocampus implies an involvement of neuropsin in neural plasticity. Since the deduced amino acid sequence of the gene contained the complete triplet (His-Asp-Ser) of the serine protease domain, the protein was postulated to have proteolytic activity. Recombinant full-length neuropsin produced in the baculovirus/insect cell system was enzymatically inactive but was readily converted to active enzyme by endoprotease processing. The activational processing of prototype neuropsin involved the specific cleavage of the Lys32-Ile33 bond near its N terminus. Native neuropsin that was purified with a purity of 1,100-fold from mouse brain had enzymatic characteristics identical to those of active-type recombinant neuropsin. Both brain and recombinant neuropsin had amidolytic activities cleaving Arg-X and Lys-X bonds in the synthetic chromogenic substrates, and the highest specific activity was found against Boc-Val-Pro-Arg-4-methylcoumaryl-7-amide. The active-type recombinant neuropsin effectively cleaved fibronectin, an extracellular matrix protein. Taken together, these results indicate that this protease, which is enzymatically novel, has significant limbic effects by ch...Continue Reading

References

May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D Gurwitz, D D Cunningham
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J P QuigleyL M Sullivan
Sep 6, 1968·Biochemical and Biophysical Research Communications·I Schechter, A Berger
Apr 20, 1967·Biochemical and Biophysical Research Communications·I Schechter, A Berger
Jun 8, 1984·Science·G Lynch, M Baudry
Jan 1, 1983·Annual Review of Physiology·J R Sanes
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·S S Twining
Mar 1, 1994·Journal of Neurobiology·Y LiuP G Nelson
Nov 1, 1995·Neuroscience Research·J SuzukiS Shiosaka
Jan 1, 1997·Annual Review of Biochemistry·C Chothia, E Y Jones
Apr 29, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Z L ChenS Yoshida
Sep 28, 1998·The European Journal of Neuroscience·Y MomotaS Shiosaka

❮ Previous
Next ❯

Citations

Jul 25, 2003·Biochemical and Biophysical Research Communications·Angeliki MagklaraEleftherios P Diamandis
Apr 10, 1999·Brain Research·K TomizawaS Yoshida
Dec 7, 2002·Life Sciences·Yoshiro TomimatsuHiroshi Nakanishi
Feb 27, 2001·Neuroscience Research·N TaniN Matsuura
Mar 29, 2001·Neuroscience Research·X P HeS Yoshida
Sep 25, 1999·Neuroscience Research·M TaniguchiS Yoshida
Feb 17, 2000·Trends in Endocrinology and Metabolism : TEM·E P DiamandisC V Obiezu
May 20, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Liu-Ying LuoEleftherios P Diamandis
Apr 22, 2011·Nature·Benjamin K AttwoodRobert Pawlak
Jun 20, 2012·Journal of Neurotrauma·Krishna Kumar VeeravalliJasti S Rao
Oct 23, 2002·Biology of Reproduction·Kazumasa Matsumoto-MiyaiSadao Shiosaka
Aug 28, 2001·Neuroreport·C Shimizu-OkabeM Fahnestock
Jun 28, 2006·Biological Chemistry·Tadaaki KishiEleftherios P Diamandis
Apr 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Martin A Bruno, A Claudio Cuello
Jun 28, 2014·Trends in Neurosciences·Peter Sonderegger, Kazumasa Matsumoto-Miyai
Sep 27, 2015·Biochimie·Magdalena KalinskaJan Potempa
Jun 8, 2011·The Journal of Physiology·Yasuyuki IshikawaSadao Shiosaka
Nov 18, 2003·The British Journal of Dermatology·T KiriharaS Shiosaka
May 18, 2006·The FEBS Journal·Loyse M FelberSylvain M Cloutier
Mar 21, 2003·Biochemical and Biophysical Research Communications·Guang W WongRichard L Stevens
Mar 29, 2001·Molecular and Cellular Neurosciences·A HirataS Shiosaka
Jun 18, 2011·Journal of Chemical Neuroanatomy·Sadao Shiosaka, Yasuyuki Ishikawa
Jul 10, 2010·Biochimie·Peter GoettigHans Brandstetter
Dec 28, 2010·Behavioural Brain Research·Edgar KornisiukDiana Jerusalinsky
Jun 22, 2016·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Arne HerringKathy Keyvani

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

© 2022 Meta ULC. All rights reserved