PMID: 9546204Apr 18, 1998Paper

Characterization of repetitive DNA sequences carrying 5S rDNA of the triploid ginbuna (Japanese silver crucian carp, Carassius auratus langsdorfi)

Genes & Genetic Systems
M Murakami, H Fujitani

Abstract

Repetitive DNA sequences (Hi-b; 209 bp in length) were isolated from the HindIII digests of the genomic DNA of the triploid ginbuna, Carassius auratus langsdorfi. Sequence analyses revealed that the Hi-b repetitive units were comprised of the complete coding regions of 5S rDNA (120 bp in size) and their 5'flanking regions. The sequences of the Hi-b units from the same individual were highly homogeneous. Southern blot hybridization to the Hi-b probe displayed intricate patterns that represented the presence of other repetitive units containing the Hi-b related sequences. A major family of repetitive sequences related to the Hi-b was then obtained by the polymerase chain reaction using asymmetry primers for the 5S coding regions. These 331-bp sequences (AZ5S's) contained 5S pseudogenes as well as the almost entire Hi-b sequences, and seemed to be the true 5S rDNAs. The tandem arrangements of the AZ5S sequences explained most of the complex results of Southern blots. Another class of intriguing repeat units (Hi-b-beta and Hi-b-gamma) were also isolated. Fluorescence in situ hybridization data revealed two major signals on a pair of homologous chromosomes and several minor signals on other chromosomes in the triploid ginbuna, indic...Continue Reading

References

Oct 12, 1978·Nature·J R Miller, G G Brownlee
Feb 1, 1992·Genome Génome / Conseil National De Recherches Canada·J S QuinnB N White
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H Endoh, N Okada
Jan 1, 1980·Annual Review of Genetics·D L Brutlag
Jan 1, 1983·Histochemistry·S Hamada, S Fujita
Jan 1, 1982·International Review of Cytology·M F Singer
May 11, 1981·Nucleic Acids Research·T D MashkovaL L Kisselev
Mar 1, 1995·The Journal of Heredity·S MizunoT Mihira
Jan 1, 1994·Cytogenetics and Cell Genetics·A M PendasE Garcia-Vazquez
Oct 1, 1993·Genomics·E R WijersJ A Lenstra
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S MurataN Okada
Jan 1, 1996·Cytogenetics and Cell Genetics·P MoranA M Pendas
Nov 1, 1991·Trends in Ecology & Evolution·N Okada

❮ Previous
Next ❯

Citations

Jul 24, 2008·Genetica·Mika FujiwaraSouichirou Kubota
Apr 12, 2007·Genome Génome / Conseil National De Recherches Canada·Marta GromichoMaria João Collares-Pereira
Oct 7, 2010·Genome Génome / Conseil National De Recherches Canada·María Ubeda-ManzanaroLaureana Rebordinos
Oct 30, 2001·Genome Génome / Conseil National De Recherches Canada·A P WaskoP M Galetti
Jul 16, 2009·Marine Biotechnology·Shaojun LiuYun Liu
Nov 23, 2006·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·H P ZhuJ F Gui
Oct 22, 2015·RNA Biology·Sergio I CastroChristian Blouin
Jan 1, 2013·Micron : the International Research and Review Journal for Microscopy·Vinicius MarquioniMarcelo de Bello Cioffi
Jan 24, 2017·Gene·Alain Victor BarrosMarcelo Ricardo Vicari
Mar 26, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A C Stone, M Stoneking
Apr 17, 2016·Science China. Life Sciences·Qinbo QinShaojun Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.