PMID: 8980521Nov 1, 1996Paper

Characterization of replication origins flanking the 23S rRNA gene in tobacco chloroplast DNA

Plant Molecular Biology
Z LuB L Nielsen

Abstract

Using 5' end-labeled nascent strands of tobacco chloroplast DNA (ctDNA) as a probe, replication displacement loop (D-loop) regions were identified. The strongest hybridization was observed with restriction fragments containing the rRNA genes from the inverted repeat region. Two-dimensional gel analysis of various digests of tobacco ctDNA suggested that a replication origin is located near each end of the 7.1 kb BamHI fragment containing part of the rRNA operon. Analysis of in vitro replication products indicated that templates from either of the origin regions supported replication, while the vector alone or ctDNA clones from other regions of the genome did not support in vitro replication. Sequences from both sides of the BamHI site in the rRNA spacer region were required for optimal in vitro DNA replication activity. Primer extension was used for the first time to identify the start site of DNA synthesis for the D-loop in the rRNA spacer region. The major 5' end of the D-loop was localized to the base of a stem-loop structure which contains the rRNA spacer BamHI site. Primer extension products were insensitive to both alkali and RNase treatment, suggesting that RNA primers had already been removed from the 5' end of nascent D...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A M Gillum, D A Clayton
Sep 1, 1975·Biochimica Et Biophysica Acta·R Kolodner, K K Tewari
Apr 11, 1992·Nucleic Acids Research·O Hyrien, M Méchali
Apr 22, 1992·Proceedings. Biological Sciences·A G MayhookH T Jacobs
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H CeruttiA T Jagendorf
Mar 1, 1992·Molecular & General Genetics : MGG·W L Chiu, B B Sears
Mar 1, 1992·Molecular & General Genetics : MGG·Y TakedaT Nagata
Jan 1, 1991·Annual Review of Cell Biology·D A Clayton
Jun 1, 1991·Plant Molecular Biology·B L NielsenK K Tewari
Aug 20, 1991·Journal of Molecular Biology·L Martín-ParrasJ B Schvartzman
Jun 1, 1990·Plant Molecular Biology·D Infante, A Weissbach
Feb 11, 1990·Nucleic Acids Research·M H Linskens, J A Huberman
Mar 1, 1988·Molecular and Cellular Biology·R MeekerK K Tewari
Jun 24, 1988·Nucleic Acids Research·N Carrillo, L Bogorad
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D D Chang, D A Clayton
Jan 1, 1985·Annual Review of Genetics·J D Palmer
Mar 15, 1994·European Journal of Biochemistry·M K ReddyK K Tewari
Nov 1, 1993·Plant Molecular Biology·L A HedrickG C Cannon
Nov 1, 1993·Plasmid·B L NielsenK K Tewari
May 1, 1993·Plant Physiology·H. Cerutti, A. T. Jagendorf
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M WuZ Q Nie
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·X W DengW Gruissem

❮ Previous
Next ❯

Citations

Dec 18, 2004·Plant Molecular Biology·Shashi KumarHenry Daniell
Aug 10, 2006·Plant Molecular Biology·Lars B Scharff, Hans-Ulrich Koop
Jan 26, 2011·Plant Molecular Biology·Matthew G LassenBrent L Nielsen
Mar 23, 2011·Plant Molecular Biology·Susann WickeDietmar Quandt
Jan 30, 2003·Molecular and Biochemical Parasitology·Divya SinghSaman Habib
Oct 18, 2002·The Plant Journal : for Cell and Molecular Biology·Stefan K MühlbauerHans-Ulrich Koop
May 2, 2007·The Plant Journal : for Cell and Molecular Biology·Lars B Scharff, Hans-Ulrich Koop
Dec 27, 2005·Journal of Molecular Biology·Medha ManchekarBrent L Nielsen
Aug 14, 2010·Acta Biochimica Et Biophysica Sinica·Huifeng ShenWanqi Liang
May 2, 1997·Journal of Molecular Biology·M KunnimalaiyaanB L Nielsen
Jul 19, 2002·Molecular Microbiology·D H WilliamsonR J M Wilson
Sep 15, 1997·Nucleic Acids Research·M Kunnimalaiyaan, B L Nielsen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.